Application
Western blot analysis of αA-crystallin expression in Y79 whole cell lysate.Application
Immunofluorescence staining of methanol-fixed Y79 cells showing cytoplasmic localization.Application
Western blot analysis of αA-crystallin expression in mouse eye (A) and rat eye (B) tissue extracts.| Product Name | αA-crystallin Antibody (FL-173) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Crystallins are the major proteins of the vertebrate eye lens, where they maintain the transparency and refractive index of the lens. Crystallins are divided into å, ∫ and © families, and the ∫- and ©-crystallins also compose a superfamily. Crystallins usually contain seven distinct protein regions, including four homologous motifs, a connecting peptide, and N- and C-terminal extensions. å-crystallins consist of three gene products, åA, åB and åC-crystallin, which are members of the small heat shock protein family (HSP 20). They are induced by heat shock, and act as molecular chaperones by holding denatured proteins in large soluble aggregates. However, unlike other molecular chaperones, å-crystallins do not renature these proteins. Expression of åA-crystallin is restricted to the lens. Defects in this gene cause autosomal dominant congenital cataracts (ADCC). The human åB-crystallin gene product is expressed in many tissues, including lens, heart and skeletal muscle. Elevated expression of åB-crystallin is associated with many neurological diseases, and a missense mutation in this gene has co-segregated in a family with a Desmin-related myopathy. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of &alphaA-crystallin and, to a lesser extent, &alphaB-crystallin of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-173 representing full length &alpha A-crystallin of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-173 representing full length αA-crystallin of human origin |
| Entrez Gene | 1409 |
|---|
Application
Western blot analysis of αA-crystallin expression in Y79 whole cell lysate.
Application
Immunofluorescence staining of methanol-fixed Y79 cells showing cytoplasmic localization.
Application
Western blot analysis of αA-crystallin expression in mouse eye (A) and rat eye (B) tissue extracts.| Application Notes | recommended for detection of &alphaA-crystallin and, to a lesser extent, &alphaB-crystallin of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
