Application
Western blot analysis of Calpain 11 expression in non-transfected: (A) and mouse Calpain 11 transfected: (B) 293 whole cell lysates.Application
Western blot analysis of Calpain 11 expression in non-transfected: (A) and human Calpain 11 transfected: (B) 293T whole cell lysates.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human testis tissue showing cytoplasmic staining of cells in seminiferus ducts at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Western blot analysis of Calpain 11 expression in non-transfected 293T: (A), human Calpain 11 transfected 293T: (B) whole cell lysates and mouse testis tissue extract (C).Application
Western blot analysis of Calpain 11 expression in mouse testis (A), rat testis (B) and mouse thymus (C) tissue extracts.| Product Name | Calpain 11 Antibody (H-60) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Calpains are calcium-activated thiol proteases. They are heterodimers with one large subunit and one small subunit. The large subunit varies between family members and can be active without the small subunit. Calpains are involved in intracellular processing of proteins. Calpain 11 is a typical calpain with four domains but, in place of the typical domain IV is a similar calmodulin-like domain that associates but does not interact with the smaller regulatory subunit. Calpain 11 is found in the testis, localizing to the spermatozoa during the later stages of meiosis. It is expressed during spermatogenesis, which suggests that during meiosis and sperm functional processes, Calpain 11 may regulate calcium dependent signal transduction events. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of Calpain 11 of mouse, human origin by WB, IP, IF, IHC(P) and ELISA | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-60 mapping at the N-terminus of Calpain 11 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-60 mapping at the N-terminus of Calpain 11 of human origin |
| Entrez Gene | 11131 |
|---|
Application
Western blot analysis of Calpain 11 expression in non-transfected: (A) and mouse Calpain 11 transfected: (B) 293 whole cell lysates.
Application
Western blot analysis of Calpain 11 expression in non-transfected: (A) and human Calpain 11 transfected: (B) 293T whole cell lysates.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human testis tissue showing cytoplasmic staining of cells in seminiferus ducts at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Western blot analysis of Calpain 11 expression in non-transfected 293T: (A), human Calpain 11 transfected 293T: (B) whole cell lysates and mouse testis tissue extract (C).
Application
Western blot analysis of Calpain 11 expression in mouse testis (A), rat testis (B) and mouse thymus (C) tissue extracts.| Application Notes | recommended for detection of Calpain 11 of mouse, human origin by WB, IP, IF, IHC(P) and ELISA |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
