Application
Western blot analysis of γ2 Tubulin expression in non-transfected: (A) and human γ2 Tubulin transfected: (B) 293T whole cell lysates.Application
Immunofluorescence staining of normal mouse intestine frozen section showing cytoplasmic staining.Application
Western blot analysis of γ Tubulin expression in HeLa (A), K-562 (B), NIH/3T3 (C), MCF7 (D) and KNRK (E) whole cell lysates.| Product Name | γ Tubulin Antibody (H-183) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Tubulin is a major cytoskeleton component that has five distinct forms, designated a, b, g, d and e tubulin. a and b tubulins form heterodimers which multimerize to form a microtubule filament. Multiple b Tubulin isoforms (b1, b2, b3, b4, b5, b6 and b8) have been characterized and are expressed in mammalian tissues. b1 and b4 are present throughout the cytosol, b2 is present in the nuclei and nucleoplasm, and b3 is a neuron-specific cytoskeletal protein. g Tubulin forms the gammasome, which is required for nucleating microtubule filaments at the centrosome. Both d Tubulin and e Tubulin are associated with the centrosome. d Tubulin is a homolog of the Chlamydomonas d Tubulin Uni3 and is found in association with the centrioles, whereas e Tubulin localizes to the pericentriolar material. e Tubulin exhibits a cell cycle-specific pattern of localization; first associating with only the older of the centrosomes in a newly duplicated pair, and later associating with both centrosomes. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of &gamma Tubulin of mouse, rat, human and Xenopus origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 269-451 mapping at the C-terminus of &gamma Tubulin of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 269-451 mapping at the C-terminus of γ Tubulin of human origin |
| Entrez Gene | 7283 |
|---|
Application
Western blot analysis of γ2 Tubulin expression in non-transfected: (A) and human γ2 Tubulin transfected: (B) 293T whole cell lysates.
Application
Immunofluorescence staining of normal mouse intestine frozen section showing cytoplasmic staining.
Application
Western blot analysis of γ Tubulin expression in HeLa (A), K-562 (B), NIH/3T3 (C), MCF7 (D) and KNRK (E) whole cell lysates.| Application Notes | recommended for detection of &gamma Tubulin of mouse, rat, human and Xenopus origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
