Application
Immunofluorescence staining of formalin-fixed Hep G2 cells showing nuclear localization.Application
Western blot analysis of Pin1 expression in PC-3 nuclear extract.Application
Western blot analysis of Pin1 expression in non-transfected: (A) and human Pin1 transfected: (B) 293T whole cell lysates and Jurkat nuclear extract (C).Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human fallopian tube tissue showing nuclear and cytoplasmic staining of glandular cells (low and high magnification). Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Western blot analysis of Pin1 expression in non-transfected: (A) and human Pin1 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of Pin1 expression in non-transfected: (A) and mouse Pin1 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of Pin1 expression in HeLa (A), K-562 (B) and Jurkat (C) nuclear extracts.| Product Name | Pin1 Antibody (H-123) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | NIMA was originally shown in Aspergillus nidulans to be necessary for entry into mitosis. NIMA-related mammalian proteins have since been identified as Nek1, Nek2 and Nek3. High expression of Nek1 is seen in male and female germ cell lines of mouse. Nek2 is the closest known mammalian relative to NIMA. Like NIMA, Nek2 expression peaks at the G2 to M phase transition. Pin1 was originally identified as a NIMA-interacting protein. Pin1 is a peptidyl-prolyl cis/trans isomerase (PPIase), which specifically binds to phosphoserine-proline or phosphothreonine-proline bonds in mitotic phosphoproteins. While previously identified PPIases have been shown to be involved in protein folding, assembly and transport, Pin1 is the first PPIase to be identified as a required protein for cell viability. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of Pin1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 41-163 mapping at the C-terminus of Pin1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 41-163 mapping at the C-terminus of Pin1 of human origin |
| Entrez Gene | 5300 |
|---|
Application
Immunofluorescence staining of formalin-fixed Hep G2 cells showing nuclear localization.
Application
Western blot analysis of Pin1 expression in PC-3 nuclear extract.
Application
Western blot analysis of Pin1 expression in non-transfected: (A) and human Pin1 transfected: (B) 293T whole cell lysates and Jurkat nuclear extract (C).
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human fallopian tube tissue showing nuclear and cytoplasmic staining of glandular cells (low and high magnification). Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Western blot analysis of Pin1 expression in non-transfected: (A) and human Pin1 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of Pin1 expression in non-transfected: (A) and mouse Pin1 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of Pin1 expression in HeLa (A), K-562 (B) and Jurkat (C) nuclear extracts.| Application Notes | recommended for detection of Pin1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
