Application
Western blot analysis of Myosin Id expression in Caco-2 whole cell lysate.Application
Western blot analysis of Myosin Id expression in Hep G2 whole cell lysate.| Product Name | Myosin Id Antibody (H-60) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Actin is a highly conserved protein that is expressed in all eukaryotic cells. Actin filaments can form both stable and labile structures and are crucial components of microvilli and the contractile apparatus of muscle cells. Troponin facilitates interaction between actin and myosin by binding to Ca2+. Troponin is made up of at least two subunits, which are divergent in cardiac muscle, fast skeletal muscle and slow skeletal muscle. Myosin is a hexamer of 2 heavy chains (MHC) and 4 light chains (MLC) that interacts with actin to generate the force for diverse cellular movements, including cytokinesis, phagocytosis and muscle contraction. Myosin Id (MYO1D) binds to calmodulin. It is expressed in most tissues, but is primarily found in brain, followed by lung and ovary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Myosin Id of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 947-1006 mapping at the C-terminus of Myosin Id of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 947-1006 mapping at the C-terminus of Myosin Id of human origin |
| Entrez Gene | 4642 |
|---|
Application
Western blot analysis of Myosin Id expression in Caco-2 whole cell lysate.
Application
Western blot analysis of Myosin Id expression in Hep G2 whole cell lysate.| Application Notes | recommended for detection of Myosin Id of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
