Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human fallopian tube tissue showing cytoskeletal staining of glandular cells.Application
Western blot analysis of Cortactin expression in non-transfected: (A) and human Cortactin transfected: (B) 293T whole cell lysates.Application
Western blot analysis of Cortactin expression in non-transfected: (A) and mouse Cortactin transfected: (B) 293T whole cell lysates.Application
Western blot analysis of Cortactin expression in HeLa whole cell lysate.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human duodenum tissue showing cytoplasmic staining of glandular cells (low and high magni?fication). Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Western blot analysis of Cortactin expression in HeLa (A) and F9 (B) whole cell lysates.Application
Western blot analysis of Cortactin expression in PC-12 whole cell lysate.Application
Immunofluorescence staining of methanol-fixed PC-12 cells showing cytoskeletal localization.| Product Name | Cortactin Antibody (H-191) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Cortactin (also designated Ems-1) is a filamentous Actin (F-Actin) binding protein that has been shown to be a substrate for Src p60. Cortactin contains tandem 37 amino acid repeats at the amino-terminus and an SH3 domain at the carboxy-terminus. The tandem repeats appear to be necessary for F-Actin binding. Tyrosine phosphorylation of Cortactin by Src p60 results in diminished F-Actin binding to Cortactin and reduced F-Actin cross-linking activity. Cortactin has also been shown to be phosphorylated in response to FGF-1. Cortactin exhibits abundant expression in megakaryocytes and platelets, and it may play a role in the maturation of megakaryocytes. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of Cortactin of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 309-499 mapping near the C-terminus of Cortactin of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 309-499 mapping near the C-terminus of Cortactin of human origin |
| Entrez Gene | 2017 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human fallopian tube tissue showing cytoskeletal staining of glandular cells.
Application
Western blot analysis of Cortactin expression in non-transfected: (A) and human Cortactin transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of Cortactin expression in non-transfected: (A) and mouse Cortactin transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of Cortactin expression in HeLa whole cell lysate.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human duodenum tissue showing cytoplasmic staining of glandular cells (low and high magni?fication). Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Western blot analysis of Cortactin expression in HeLa (A) and F9 (B) whole cell lysates.
Application
Western blot analysis of Cortactin expression in PC-12 whole cell lysate.
Application
Immunofluorescence staining of methanol-fixed PC-12 cells showing cytoskeletal localization.| Application Notes | recommended for detection of Cortactin of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
