Application
Western blot analysis of β-catenin expression in A-431 (A) and SK-BR-3 (B) whole cell lysates.Application
Western blot analysis of β-catenin expression in SH-SY5Y (A) and NIH/3T3 (B) whole cell lysates.Application
Immunofluorescence staining of formalin-fixed Hep G2 cells showing membrane localization.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human cervix tissue showing membrane staining of squamous epithelial cells.Application
Antibodies tested include p-β-catenin (Ser 33)-R: (A,B,C) and β-catenin (H-102): (D,E,F).Application
Western blot analysis of β-catenin expression in non-transfected: (A) and human β-catenin transfected: (B) 293T whole cell lysates.Application
Immunofluorescence staining of normal mouse skin frozen section showing membrane staining.Application
Control Input (A). Antibodies used included TRβ1 (J51): and TRβ1 (J52): (B), p300 (C-20): , p300 (H-272): and p300 (N-15): (C), β-catenin (H-102): , β-catenin (C-18): and β-catenin (E-5): (D). Data kindly provided by M.G. Rosenfeld and reproduced wtih permission from Gold et al., Neuron 2003, 40: 1119-1131.Application
Antibodies tested include HDAC1 (H-15): , HDAC1 (C-19): and HDAC1 (H-11): (A), β-catenin (H-120): , β-catenin (C-18): and β-catenin (E-5): (B), E2F-4 (A-20): , E2F-4 (D-3): , E2F-4 (RK-13): and E2F-4 (C-20): (C), and p130 (C-20): , p130 (211.6): and p130 (H-125): (D). Data kindly provided by M.G. Rosenfeld and reproduced with permission from Proc. Natl. Acad. Sci. 100: 3245-3250. Copyright 2003 National Academy of Sciences, USA.Application
Antibodies tested include β-catenin (H-102): , β-catenin (C-18): , β-catenin (E-5): , Tip60 (N-17): , TRRAP (T-17): , TRRAP (Y-18): , TRRAP (F-20): , TRRAP (H-300): , CBP (A-22): , CBP (C-20): , CBP (451): , CPB (C-1): , p300 (H-272): , p300 (N-15): and p300 (C-20): . Data kindly provided by M.G. Rosenfeld and reproduced with permission from Kioussi et al., Cell 2002, 111: 673-685.Application
Antibodies tested include LEF-1 (C-19): amd LEF-1 (N-17): (B), β-catenin (H-102): , β-catenin (C-18): and β-catenin (E-5): (C), HDAC1 (H-11): , HDAC1 (C-19): and HDAC1 (H-11): (D), HDAC2 (C-8): , HDAC2 (H-54): and HDAC2 (C-19): (E). Input control (A). Data kindly provided by M.G. Rosenfeld and reproduced with permission from Kioussi et al., Cell 2002, 111: 673-685.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast tumor showing membrane staining.Application
Western blot analysis of β-catenin expression in HeLa (A) and MCF7 (B) whole cell lysates.| Product Name | β-catenin Antibody (H-102) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | The catenins, å, ∫ and ©, are proteins which bind to the highly conserved, intracellular cytoplasmic tail of E-cadherin. Together, the catenin/cadherin complexes play an important role mediating cellular adhesion. å-catenin was initially described as an E-cadherin associated protein, and since has been shown to associate with other members of the cadherin family, such as N-cadherin and P-cadherin. ∫-catenin associates with the cytoplasmic portion of E-cadherin, which is necessary for the function of E-cadherin as an adhesion molecule. ∫-catenin has also been found in complexes with the tumor suppressor protein APC (5). ©-catenin, also known as plakoglobin, binds with å-catenin and N-cadherin. It has been shown that the transmembrane phosphatase PTPµ associates with catenin/cadherin complexes and may regulate complex signaling. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of &beta-catenin of mouse, rat, human, zebrafish and Xenopus origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 680-781 mapping at the C-terminus of &beta -catenin of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 680-781 mapping at the C-terminus of β-catenin of human origin |
| Entrez Gene | 1499 |
|---|
Application
Western blot analysis of β-catenin expression in A-431 (A) and SK-BR-3 (B) whole cell lysates.
Application
Western blot analysis of β-catenin expression in SH-SY5Y (A) and NIH/3T3 (B) whole cell lysates.
Application
Immunofluorescence staining of formalin-fixed Hep G2 cells showing membrane localization.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human cervix tissue showing membrane staining of squamous epithelial cells.
Application
Antibodies tested include p-β-catenin (Ser 33)-R: (A,B,C) and β-catenin (H-102): (D,E,F).
Application
Western blot analysis of β-catenin expression in non-transfected: (A) and human β-catenin transfected: (B) 293T whole cell lysates.
Application
Immunofluorescence staining of normal mouse skin frozen section showing membrane staining.
Application
Control Input (A). Antibodies used included TRβ1 (J51): and TRβ1 (J52): (B), p300 (C-20): , p300 (H-272): and p300 (N-15): (C), β-catenin (H-102): , β-catenin (C-18): and β-catenin (E-5): (D). Data kindly provided by M.G. Rosenfeld and reproduced wtih permission from Gold et al., Neuron 2003, 40: 1119-1131.
Application
Antibodies tested include HDAC1 (H-15): , HDAC1 (C-19): and HDAC1 (H-11): (A), β-catenin (H-120): , β-catenin (C-18): and β-catenin (E-5): (B), E2F-4 (A-20): , E2F-4 (D-3): , E2F-4 (RK-13): and E2F-4 (C-20): (C), and p130 (C-20): , p130 (211.6): and p130 (H-125): (D). Data kindly provided by M.G. Rosenfeld and reproduced with permission from Proc. Natl. Acad. Sci. 100: 3245-3250. Copyright 2003 National Academy of Sciences, USA.
Application
Antibodies tested include β-catenin (H-102): , β-catenin (C-18): , β-catenin (E-5): , Tip60 (N-17): , TRRAP (T-17): , TRRAP (Y-18): , TRRAP (F-20): , TRRAP (H-300): , CBP (A-22): , CBP (C-20): , CBP (451): , CPB (C-1): , p300 (H-272): , p300 (N-15): and p300 (C-20): . Data kindly provided by M.G. Rosenfeld and reproduced with permission from Kioussi et al., Cell 2002, 111: 673-685.
Application
Antibodies tested include LEF-1 (C-19): amd LEF-1 (N-17): (B), β-catenin (H-102): , β-catenin (C-18): and β-catenin (E-5): (C), HDAC1 (H-11): , HDAC1 (C-19): and HDAC1 (H-11): (D), HDAC2 (C-8): , HDAC2 (H-54): and HDAC2 (C-19): (E). Input control (A). Data kindly provided by M.G. Rosenfeld and reproduced with permission from Kioussi et al., Cell 2002, 111: 673-685.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast tumor showing membrane staining.
Application
Western blot analysis of β-catenin expression in HeLa (A) and MCF7 (B) whole cell lysates.| Application Notes | recommended for detection of &beta-catenin of mouse, rat, human, zebrafish and Xenopus origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
