Application
Intracellular FCM analysis of fixed and permeabilized control (green line histogram) and TRAF3 transfected (solid orange histogram) IB4 cells. Dotted pink histogram represents the isotype control, normal rabbit IgG: .Application
Immunofluorescence staining of methanol-fixed TRAF3-transfected COS cells showing cytoplasmic localization.Application
Antibodies tested include TRAF3 (G-6): (A) and TRAF3 (H-122): (B).Application
Western blot analysis of TRAF3 expression in TRAF3 transfected IB4 cells.| Product Name | TRAF3 Antibody (H-122) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Tumor necrosis factor (TNF)-activated cell signaling is mediated primarily through the TNF receptor 1 (TNF-R1) and, to a lesser extent, TNF-R2. Both TNF receptors are members of the expanding TNF receptor superfamily which includes the FAS antigen and CD40. Potential insight into an understanding of TNF receptor-mediated signaling was provided by the identification of two related proteins, TRAF1 and TRAF2 (for TNF receptor-associated factors 1 and 2, respectively). Both function to form heterodimeric complexes and associate with the cytoplasmic domain of TNF-R2. A third member of this protein family, alternatively designated CD40 bp, CRAF1, LAP1 or TRAF3, has been identified and shown to associate with the cytoplasmic domain of CD40. The similarity between a specific region of TRAF3 with regions of TRAF1 and TRAF2 define a “TRAF-C” domain that is necessary and sufficient for CD40 binding and homodimerization. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFFCELISA |
| recommended for detection of TRAF3 of mouse, rat and human origin by WB, IP, IF, FCM and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 322-444 of TRAF3 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 322-444 of TRAF3 of human origin |
| Entrez Gene | 7187 |
|---|
Application
Intracellular FCM analysis of fixed and permeabilized control (green line histogram) and TRAF3 transfected (solid orange histogram) IB4 cells. Dotted pink histogram represents the isotype control, normal rabbit IgG: .
Application
Immunofluorescence staining of methanol-fixed TRAF3-transfected COS cells showing cytoplasmic localization.
Application
Antibodies tested include TRAF3 (G-6): (A) and TRAF3 (H-122): (B).
Application
Western blot analysis of TRAF3 expression in TRAF3 transfected IB4 cells.| Application Notes | recommended for detection of TRAF3 of mouse, rat and human origin by WB, IP, IF, FCM and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
