Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human lung tissue showing cytoplasmic staining of respiratory epithelial cells.Application
Intracellular FCM analysis of fixed and permeabilized control (green line histogram) and TRAF3 transfected (solid orange histogram) IB4 cells. Dotted pink histogram represents the isotype control, normal goat IgG: .Application
Immunofluorescence staining of methanol-fixed TRAF3-transfected NIH/3T3 cells showing cytoplasmic localization.Application
Western blot analysis of TRAF3 expression in control (A) and TRAF3 transfected (B) whole cell lysates.| Product Name | TRAF3 Antibody (H-20) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Tumor necrosis factor (TNF)-activated cell signaling is mediated primarily through the TNF receptor 1 (TNF-R1) and, to a lesser extent, TNF-R2. Both TNF receptors are members of the expanding TNF receptor superfamily which includes the FAS antigen and CD40. Potential insight into an understanding of TNF receptor-mediated signaling was provided by the identification of two related proteins, TRAF1 and TRAF2 (for TNF receptor-associated factors 1 and 2, respectively). Both function to form heterodimeric complexes and associate with the cytoplasmic domain of TNF-R2. A third member of this protein family, alternatively designated CD40 bp, CRAF1, LAP1 or TRAF3, has been identified and shown to associate with the cytoplasmic domain of CD40. The similarity between a specific region of TRAF3 with regions of TRAF1 and TRAF2 define a “TRAF-C” domain that is necessary and sufficient for CD40 binding and homodimerization. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCFCELISA |
| recommended for detection of TRAF3 of mouse, rat and human origin by WB, IP, IF, IHC(P), FCM and ELISA also reactive with additional species, including equine and canine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the N-terminus of TRAF3 of human origin |
|---|---|
| Tissue Specificity | epitope mapping at the N-terminus of TRAF3 of human origin |
| Entrez Gene | 7187 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human lung tissue showing cytoplasmic staining of respiratory epithelial cells.
Application
Intracellular FCM analysis of fixed and permeabilized control (green line histogram) and TRAF3 transfected (solid orange histogram) IB4 cells. Dotted pink histogram represents the isotype control, normal goat IgG: .
Application
Immunofluorescence staining of methanol-fixed TRAF3-transfected NIH/3T3 cells showing cytoplasmic localization.
Application
Western blot analysis of TRAF3 expression in control (A) and TRAF3 transfected (B) whole cell lysates.| Application Notes | recommended for detection of TRAF3 of mouse, rat and human origin by WB, IP, IF, IHC(P), FCM and ELISA also reactive with additional species, including equine and canine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
