Application
Immunofluorescence staining of methanol-fixed NIH/3T3 cells showing cytoplasmic localization.Application
Western blot analysis of 14-3-3 θ expression in non-transfected 293T: (A), human 14-3-3 θ transfected 293T: (B), PC-12 (C), HeLa (D) and A-431 (E) whole cell lysates and mouse placenta tissue extract (F).Application
Western blot analysis of 14-3-3 θ expression in non-transfected 293T: (A), human 14-3-3 θ transfected 293T: (B) and NIH/3T3 (C) whole cell lysates.Application
Western blot analysis of 14-3-3 θ expression in non-transfected: (A) and mouse 14-3-3 θ transfected: (B) 293T whole cell lysates.Application
Western blot analysis of 14-3-3 θ expression in HeLa whole cell lysate (A) and mouse prostate tissue extract (B).Application
Western blot analysis of 14-3-3 θ expression in non-transfected: (A) and human 14-3-3 θ transfected: (B) 293T whole cell lysates.Application
Western blot analysis of 14-3-3 θ expression in NIH/3T3 whole cell lysate.Application
Western blot analysis of 14-3-3 θ expression in non-transfected: (A) and human 14-3-3 θ transfected: (B) 293T whole cell lysates.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human prostate cancer tissue showing cytoplasmic staining of tumor cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Indirect, intracellular FCM analysis of fixed and permeabilized K-562 cells stained with 14-3-3 θ (C-17), followed by PE-conjugated goat anti-rabbit IgG: . Black line histogram represents the isotype control, normal rabbit IgG: .Application
Indirect, intracellular FCM analysis of fixed and permeabilized K-562 cells stained with 14-3-3 θ (C-17), followed by FITC-conjugated goat anti-rabbit IgG: . Black line histogram represents the isotype control, normal goat IgG: .Application
Western blot analysis of 14-3-3 θ expression in NIH/3T3 (A), KNRK (B), HeLa (C) and A-431 (D) whole cell lysates.Application
Immunofluorescence staining of methanol-fixed A-431 cells showing cytoplasmic staining.| Product Name | 14-3-3 θ Antibody (C-17) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | 14-3-3 proteins regulate many cellular processes relevant to cancer biology, notably apoptosis, mitogenic signaling and cell-cycle checkpoints. Seven isoforms comprise this family of signaling intermediates, denoted 14-3-3 ∫, ©, é, Ω, ñ, † and s. 14-3-3 proteins form dimers that present two binding sites for ligand proteins, thereby bringing together two proteins that may not otherwise associate. These ligands largely share a 14-3-3 consensus binding motif and exhibit serine/threonine phosphorylation. 14-3-3 proteins function in broad regulation of these ligand proteins, by cytoplasmic sequestration, occupation of interaction domains and import/export sequences, prevention of degradation, activation/repression of enzymatic activity and facilitation of protein modification, and thus loss of expression contributes to a vast array of pathogenic cellular activities. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCFCELISA |
| recommended for detection of 14-3-3 &theta of mouse, rat and human origin by WB, IP, IF, IHC(P), ELISA and indirect FCM using PE (sc-3739)- or FITC (sc-2012)-conjugated goat anti-rabbit IgG also reactive with additional species, including equine, canine, bovine, porcine and avian | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping near the C-terminus of 14-3-3 &theta of rat origin |
|---|---|
| Tissue Specificity | epitope mapping near the C-terminus of 14-3-3 θ of rat origin |
| Entrez Gene | 10971 |
|---|
Application
Immunofluorescence staining of methanol-fixed NIH/3T3 cells showing cytoplasmic localization.
Application
Western blot analysis of 14-3-3 θ expression in non-transfected 293T: (A), human 14-3-3 θ transfected 293T: (B), PC-12 (C), HeLa (D) and A-431 (E) whole cell lysates and mouse placenta tissue extract (F).
Application
Western blot analysis of 14-3-3 θ expression in non-transfected 293T: (A), human 14-3-3 θ transfected 293T: (B) and NIH/3T3 (C) whole cell lysates.
Application
Western blot analysis of 14-3-3 θ expression in non-transfected: (A) and mouse 14-3-3 θ transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of 14-3-3 θ expression in HeLa whole cell lysate (A) and mouse prostate tissue extract (B).
Application
Western blot analysis of 14-3-3 θ expression in non-transfected: (A) and human 14-3-3 θ transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of 14-3-3 θ expression in NIH/3T3 whole cell lysate.
Application
Western blot analysis of 14-3-3 θ expression in non-transfected: (A) and human 14-3-3 θ transfected: (B) 293T whole cell lysates.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human prostate cancer tissue showing cytoplasmic staining of tumor cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Indirect, intracellular FCM analysis of fixed and permeabilized K-562 cells stained with 14-3-3 θ (C-17), followed by PE-conjugated goat anti-rabbit IgG: . Black line histogram represents the isotype control, normal rabbit IgG: .
Application
Indirect, intracellular FCM analysis of fixed and permeabilized K-562 cells stained with 14-3-3 θ (C-17), followed by FITC-conjugated goat anti-rabbit IgG: . Black line histogram represents the isotype control, normal goat IgG: .
Application
Western blot analysis of 14-3-3 θ expression in NIH/3T3 (A), KNRK (B), HeLa (C) and A-431 (D) whole cell lysates.
Application
Immunofluorescence staining of methanol-fixed A-431 cells showing cytoplasmic staining.| Application Notes | recommended for detection of 14-3-3 &theta of mouse, rat and human origin by WB, IP, IF, IHC(P), ELISA and indirect FCM using PE (sc-3739)- or FITC (sc-2012)-conjugated goat anti-rabbit IgG also reactive with additional species, including equine, canine, bovine, porcine and avian |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
