Application
Western blot analysis of IL-2Rα expression in non-transfected: (A) and human IL-2Rα transfected: (B) 293T whole cell lysates.| Product Name | IL-2Rα Antibody (FL-272) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | The IL-2 receptor is a multicomponent complex consisting of three subunits, å, ∫ and ©, each of which is required for high-affinity binding of IL-2. The å chain functions primarily in binding IL-2, whereas the ∫ and © chains contribute to IL-2 binding and are essential to IL-2-induced activation of signaling pathways leading to T cell growth. Both IL-4R and IL-7R were initially described as single chain, high-affinity, ligand-binding cytokine receptors. However, it is now well-established that the IL-2R© chain functions as a second subunit of the high-affinity IL-4R and IL-7R receptors. Consequently, the originally described subunits of these latter receptors are now referred to as IL-4Rå and IL-7Rå, respectively, while the common subunit is referred to as ©c. Although the common © chain enhances ligand binding in these three cytokine receptors, it has no capacity to bind these ligands on its own. There is evidence that the ©c chain is also a subunit of IL-13R. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of IL-2R&alpha of human and, to a lesser extent, mouse and rat origin by WB, IP, IF and ELISA | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-272 representing full length IL-2R&alpha of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-272 representing full length IL-2Rα of human origin |
| Entrez Gene | 3559 |
|---|
Application
Western blot analysis of IL-2Rα expression in non-transfected: (A) and human IL-2Rα transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of IL-2R&alpha of human and, to a lesser extent, mouse and rat origin by WB, IP, IF and ELISA |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
