Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human rectum tissue showing nuclear and cytoplasmic staining of glandular cells.Application
Immunofluorescence staining of formalin-fixed Hep G2 cells showing nuclear and cytoplasmic localization.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human pancreas tissue showing nuclear staining of glandular cells.Application
Western blot analysis of TIM expression in Sol8 whole cell lysate.Application
Western blot analysis of TIM expression in non-transfected: (A) and human TIM transfected: (B) 293T whole cell lysates and HeLa nuclear extract (C).Application
Immunofluorescence staining of normal mouse intestine frozen section showing cytoplasmic staining.| Product Name | TIM Antibody (FL-249) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Glycolysis is an evolutionarily conserved series of ten chemical reactions that utilizes eleven enzymes to concomitantly generate pyruvate and ATP from glucose. Triosephosphate isomerase, known as TIM or TPI, is ubiquitously expressed and catalyzes the interconversion of dihydroxyacetone phosphate (DHAP) and glyceraldehyde-3-phosphate in the glycolitic pathway. The human TIM gene spans 3.5 kilobase pairs, contains seven exons and encodes a 249 amino acid protein. The TIM promoter element contains a TATA box (positions -27 to -21) and multiple GC boxes (positions -126 to -48) that variably conform to the consensus Sp1-binding site. The GC boxes function in cis to the TATA box to control both the frequency and position of transcription initiation. Deficiencies in TIM result in a rare autosomal recessive condition where a metabolic block in glycolysis and accumulating DHAP in erythrocytes can lead to non-spherocytic hemolytic anemia, recurrent infections, cardiomyopathy, and neuromuscular dysfunctions. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of TIM of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-249 representing full length TIM of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-249 representing full length TIM of human origin |
| Entrez Gene | 7167 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human rectum tissue showing nuclear and cytoplasmic staining of glandular cells.
Application
Immunofluorescence staining of formalin-fixed Hep G2 cells showing nuclear and cytoplasmic localization.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human pancreas tissue showing nuclear staining of glandular cells.
Application
Western blot analysis of TIM expression in Sol8 whole cell lysate.
Application
Western blot analysis of TIM expression in non-transfected: (A) and human TIM transfected: (B) 293T whole cell lysates and HeLa nuclear extract (C).
Application
Immunofluorescence staining of normal mouse intestine frozen section showing cytoplasmic staining.| Application Notes | recommended for detection of TIM of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
