Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human seminal vesicle tissue showing cytoplasmic staining of glandular cells.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human esophagus tissue showing cytoplasmic staining of squamous epithelial cells.Application
Western blot analysis of Myosin VI expression in H4 (A) and DU 145 (B) whole cell lysates.| Product Name | Myosin VI Antibody (H-215) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Myosin VI (MYO6), a molecular motor involved in intracellular vesicle and organelle transport, is the only Myosin motor that binds to the pointed end of Actin. This unique Myosin has only one light chain in the lever-arm domain and has highly irregular stepping with a wide range of step sizes, unlike that of other characterized Myosins. It associates with Clathrin-coated vesicles and disabled 2, indicating a role for Myosin VI in endocytosis. Mouse Myosin VI is expressed within the sensory hair cells of the cochlea. Human Myosin VI is mapped to the centromeric region of chromosome 6, a region that shows syntenic homology with the corresponding mouse chromosome 9 region, where the Snell’s Waltzer mutation is located. The behavioral effects of the mouse Snell’s Waltzer mutation are lack of responsiveness to sound, hyperactivity, head tossing and circling, due to the disorganization and fusing of stereocilia bundles within the inner ear. Defects of Myosin VI cause autosomal dominant nonsyndromic sensori-neural deafness in humans. Human Myosin VI is expressed in fetal cochlea and brain, as well as in adult brain. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of Myosin VI of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1071-1285 mapping at the C-terminus of Myosin VI of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1071-1285 mapping at the C-terminus of Myosin VI of human origin |
| Entrez Gene | 4646 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human seminal vesicle tissue showing cytoplasmic staining of glandular cells.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human esophagus tissue showing cytoplasmic staining of squamous epithelial cells.
Application
Western blot analysis of Myosin VI expression in H4 (A) and DU 145 (B) whole cell lysates.| Application Notes | recommended for detection of Myosin VI of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
