Application
Western blot analysis of PI 4-kinase α expression in HL-60 whole cell lysates.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human placenta tissue showing cytoplasmic staining of trophoblastic cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Immunofluorescence staining of normal mouse liver frozen section showing membrane and cytoplasmic staining.Application
Western blot analysis of human recombinant PI 4-kinase α fusion protein.| Product Name | PI 4-kinase α Antibody (H-142) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | The members of the phosphatidylinositol kinase (PIK) superfamily can be divided into three groups based on their substrate specificity. PIKs convert phosphatidylinositol (PI) into PI phosphate [PI(3)P], PI phosphate [PI(4)P], PI bisphosphate [PI(4,5)P2] and PI triphosphate [PI(3,4,5)P3]. The first group, the PI 3-kinases, is composed of highly related proteins designated p110å, p110∫, p110© and p110∂ which convert PI into PI(3)P and PI(4,5)P2 into PI(3,4,5)P3. The second group, the PI 4-kinases, convert PI into PI(4)P. The third group, the PI(4)P5-kinases, convert PI(4)P into PI(4,5)P2. Phosphatidylinositides represent important regulatory molecules and are involved in a diverse array of signaling pathways. Phosphatidylinositol biphosphate acts as an activator of PKCs and as a substrate for PLC ©, which converts the molecule into the second messengers, inositol-1,4,5 triphosphate and 1,2-diacylglycerol. PI(3,4,5)P3 has been shown to activate the PKC Ω isoform. Wortmannin, originally described as a specific inhibitor of PI 3-kinases, may actually be a broad spectrum inhibitor of PI kinase activity. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of PI 4-kinase &alpha of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine and canine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1300-1441 mapping within an internal region of PI 4-kinase &alpha of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1300-1441 mapping within an internal region of PI 4-kinase α of human origin |
| Entrez Gene | 5297 |
|---|
Application
Western blot analysis of PI 4-kinase α expression in HL-60 whole cell lysates.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human placenta tissue showing cytoplasmic staining of trophoblastic cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Immunofluorescence staining of normal mouse liver frozen section showing membrane and cytoplasmic staining.
Application
Western blot analysis of human recombinant PI 4-kinase α fusion protein.| Application Notes | recommended for detection of PI 4-kinase &alpha of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine and canine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
