Application
Immunofluorescence staining of methanol-fixed HeLa cells showing membrane localization.Application
Western blot analysis of SphK1 expression in C2C12 (A), PC-12 (B) and RAW 264.7 (C) whole cell lysates and rat skeletal muscle (D) and mouse embryo (E) tissue extracts.| Product Name | SphK1 Antibody (M-209) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Sphingosine kinase (SphK or SphK1) is a key enzyme catalyzing the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP or S1P). SPP is a bioactive lipid that exerts multiple biological effects in a large variety of cell types, acting as either an intracellular messenger or an extracellular ligand coupled to Edg-family receptors. Competitive inhibitors of SphK1 block formation of SPP and selectively inhibit cellular proliferation induced by a variety of factors. One potent inhibitor of SphK1 activity is DMS (N,N-dimethylsphingosine). SPP/SphK1 has been implicated as a signaling pathway that regulates diverse cellular functions, including cell growth, proliferation and survival. Specifically, SphK1 is involved in the signaling pathway(s) that protects human hepatocytes from the apoptotic action of TNFå. Furthermore, SPP/SphK1 may play an important role in neuronal survival by regulating activation of SAPKs and caspases. SphK1 is widely expressed with highest levels in adult liver, kidney, heart and skeletal muscle; however, activation of SphK1 disengages cells from their liver-specific phenotype. SphK1 is highly homologous with SphK2, another member of a growing class of sphingolipid kinases. Expression of SphK2 mRNA exhibits a markedly different tissue distribution than that of SphK1 and appears at a later stage in embryonic development. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of SphK1 of mouse, rat and human origin by WB, IP, IF and ELISA | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 125-333 mapping near the C-terminus of SphK1 of mouse origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 125-333 mapping near the C-terminus of SphK1 of mouse origin |
| Entrez Gene | 8877 |
|---|
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing membrane localization.
Application
Western blot analysis of SphK1 expression in C2C12 (A), PC-12 (B) and RAW 264.7 (C) whole cell lysates and rat skeletal muscle (D) and mouse embryo (E) tissue extracts.| Application Notes | recommended for detection of SphK1 of mouse, rat and human origin by WB, IP, IF and ELISA |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
