Application
Western blot analysis of Emt expression in human PBL whole cell lysate.| Product Name | Emt Antibody (M-109) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | The Tec family of non-receptor tyrosine kinases is composed of six proteins designated Tec, Emt (also known as Itk or Tsk), Btk (previously known as Atk, BPK or Emb), Bmx, Txk (also known as Rlk) and Dsrc28C (1–6). All members of the family contain SH3 and SH2 domains and, with the exception of Txk and Dsrc28C, also contain a pleckstrin homology (PH) and a Tec homology (TH) domain in their amino termini (2,3). Four alternatively spliced forms of Tec are found to be expressed broadly in cells of hematopoietic lineage and hepatocytes (2,3). The Emt gene product associates with CD28 and becomes activated subsequent to CD28 ligation (4). Btk is necessary for proper B cell development, and mutations in the gene encoding Btk have been associated with families suffering from X-linked agammaglobulinemia, also referred to as Bruton’s disease (5). The Bmx protein shares a high degree of homology with Btk and seems to be expressed at highest levels in the heart (6). Txk expression is T cell-specific, while expression of the Drosophila Tec homolog, Dsrc28C, is developmentally regulated (2). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Emt of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | raised against amino acids 1-109 mapping at the N-terminus of Emt of mouse origin |
|---|---|
| Tissue Specificity | raised against amino acids 1-109 mapping at the N-terminus of Emt of mouse origin |
| Entrez Gene | 3702 |
|---|
Application
Western blot analysis of Emt expression in human PBL whole cell lysate.| Application Notes | recommended for detection of Emt of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
