Application
Western blot analysis of PKA Iβ expression in non-transfected 293T: (A), mouse PKA Iβ reg transfected 293T: (B) and SW-13 (C) whole cell lysates.Application
Western blot analysis of PKA Iβ reg expression in non-transfected: (A) and human PKA Iβ reg transfected: (B) 293T whole cell lysates.Application
Western blot analysis of PKA Iβ reg expression in non-transfected: (A) and human PKA Iβ reg transfected: (B) 293T whole cell lysates and mouse brain tissue extract (C).Application
Western blot analysis of PKA Iβ regulatory subunit expression in mouse brain extract.| Product Name | PKA Iβ reg Antibody (C-19) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | The second messenger cyclic AMP (cAMP) mediates diverse cellular responses to external signals such as proliferation, ion transport, regulation of metabolism and gene transcription by activation of the cAMP-dependent protein kinase (cAPK or PKA). Activation of PKA occurs when cAMP binds to the two regulatory subunits of the tetrameric PKA holoenzyme resulting in release of active catalytic subunits. Three catalytic (C) subunits have been identified, designated Cα, Cβ and Cγ, that each represent specific gene products. Cα and Cβ are closely related (93% amino acid sequence similarity), whereas Cγ displays 83% and 79% similarity to Cα and Cβ, respectively. Activation of transcription upon elevation of cAMP levels results from trans- location of PKA to the nucleus where it phosphorylates the transcription factor cAMP response element binding protein (CREB) on serine 133 which in turn leads to TFIIB binding to TATA-box-binding protein TBP1, thus linking phospho-CREB to the pol II transcription initiation complex. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of the PKA type I&beta regulatory subunit of mouse, rat and human origin by WB, IP, IF and ELISA partially cross-reactive with type I&alpha subunit also reactive with additional species, including equine, canine, bovine, porcine and avian | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the C-terminus of PKA I&beta reg of human origin |
|---|---|
| Tissue Specificity | epitope mapping at the C-terminus of PKA Iβ reg of human origin |
| Entrez Gene | 5575 |
|---|
Application
Western blot analysis of PKA Iβ expression in non-transfected 293T: (A), mouse PKA Iβ reg transfected 293T: (B) and SW-13 (C) whole cell lysates.
Application
Western blot analysis of PKA Iβ reg expression in non-transfected: (A) and human PKA Iβ reg transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of PKA Iβ reg expression in non-transfected: (A) and human PKA Iβ reg transfected: (B) 293T whole cell lysates and mouse brain tissue extract (C).
Application
Western blot analysis of PKA Iβ regulatory subunit expression in mouse brain extract.| Application Notes | recommended for detection of the PKA type I&beta regulatory subunit of mouse, rat and human origin by WB, IP, IF and ELISA partially cross-reactive with type I&alpha subunit also reactive with additional species, including equine, canine, bovine, porcine and avian |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
