Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human fallopian tube tissue showing cytoplasmic and nuclear staining of glandular cells.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear and cytoplasmic localization (A). Immunoperoxidase staining of formalin-fixed, paraffin-embedded mouse embryo tissue showing nuclear localization (B).Application
Western blot analysis of casein kinase IIβ expression in HeLa (A), MCF7 (B), Hep G2 (C) and K-562 (D) whole cell lysates.| Product Name | casein kinase IIβ Antibody (FL-215) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Casein kinase I (also designated CKI) and casein kinase II (CKII) compose a family of serine/threonine protein kinases which are present in all eukaryotes examined to date. Casein kinase I family members, which include casein kinase Iå, I©, I∂ and Ie, have been implicated in the control of cytoplasmic and nuclear processes, including DNA replication and repair. Casein kinase II is usually expressed as a tetrameric complex consisting of either an å2∫2 or an åå'∫2 structure. The a catalytic subunit is stimulated by the ∫ regulatory subunit, which undergoes autophosphorylation. Casein kinase II activity is high in the cytosol and nucleus of proliferating and differentiating cells. Casein kinase II is known to phosphorylate more than 100 different substrates including nuclear oncoproteins, transcription factors and enzymes involved in DNA metabolism. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of casein kinase II&beta of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-215 representing full length casein kinase II&beta of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-215 representing full length casein kinase IIβ of human origin |
| Entrez Gene | 1460 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human fallopian tube tissue showing cytoplasmic and nuclear staining of glandular cells.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear and cytoplasmic localization (A). Immunoperoxidase staining of formalin-fixed, paraffin-embedded mouse embryo tissue showing nuclear localization (B).
Application
Western blot analysis of casein kinase IIβ expression in HeLa (A), MCF7 (B), Hep G2 (C) and K-562 (D) whole cell lysates.| Application Notes | recommended for detection of casein kinase II&beta of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
