Application
Antibodies tested include p-c-Jun (Ser 73)-R: (A,B,C) and c-Jun (H-79): (D,E,F).Application
Antibodies tested include p-c-Jun (KM-1): (A,B,C) and c-Jun (H-79): (D,E,F).Application
Antibodies tested include p-c-Jun (Ser 63/73)-R: (A,B,C) and c-Jun (H-79): (D,E,F).Application
Western blot analysis of c-Jun expression in non-transfected CHO: (A), human c-Jun transfected CHO: (B) and NIH/3T3 (C) whole cell lysates.Application
Western blot analysis of c-Jun expression in non-transfected 293T: (A), mouse c-Jun transfected 293T: (B) and NIH/3T3 (C) whole cell lysates.Application
Western blot analysis of c-Jun expression in non-transfected 293T: (A), mouse c-Jun transfected 293T: (B) and NIH/3T3 (C) whole cell lysates.Application
Western blot analysis of c-Jun expression in non-transfected: (A) and mouse c-Jun transfected: (B) 293T whole cell lysates.Application
Western blot analysis of c-Jun expression in non-transfected: (A) and human c-Jun transfected: (B) 293 whole cell lysates.Application
Western blot analysis of c-Jun expression in non-transfected (A) and human c-Jun transfected (B) CHO whole cell lysates.Application
Intracellular FCM analysis of fixed and permeabilized control (green line histogram) and PMA-induced (solid orange histogram) NIH/3T3 cells. Dotted pink histogram represents the isotype control, normal rabbit IgG: .Application
Antibodies tested include c-Jun (H-79): (A,B), p-c-Jun (Ser 73)-R: (C,D) and p-c-Jun (Ser 63/73)-R: (E,F).Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded normal human prostate tissue (A) and immunofluorescence staining of methanol-fixed NIH/3T3 cells (B) showing nuclear localization.Application
Western blot analysis of c-Jun p39 expression in untreated (A) and phorbol ester-induced (B) NIH/3T3 nuclear extracts.| Product Name | c-Jun Antibody (H-79) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Genes belonging to the Jun and Fos oncogene families encode nuclear proteins that are associated with a number of transcriptional complexes. The c-Jun protein is a major component of the transcription factor AP-1, originally shown to mediate phorbol ester tumor promoter (TPA)-induced expression of responsive genes through the TPA-response element (TRE). The Jun proteins form homo- and heterodimers which bind the TRE, while Fos proteins are active only as heterodimers with any of the Jun proteins. Fos/Jun heterodimers have a much higher affinity for the TRE than Jun homodimers. Ha-Ras augments c-Jun activity and stimulates phosphorylation of its activation domain. An inhibitor of Fos/Jun function, termed IP-1, associates with Fos and Jun and is inactivated upon phosphorylation induced by the cAMP-dependent protein kinase A (PKA). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCFCELISAGSChIP |
| recommended for detection of c-Jun p39 of mouse, rat, human and Xenopus origin by WB, IP, IF, IHC(P), FCM and ELISA also reactive with additional species, including canine, bovine and porcine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-79 mapping at the N-terminus of c-Jun p39 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-79 mapping at the N-terminus of c-Jun p39 of human origin |
| Entrez Gene | 3725 |
|---|
Application
Antibodies tested include p-c-Jun (Ser 73)-R: (A,B,C) and c-Jun (H-79): (D,E,F).
Application
Antibodies tested include p-c-Jun (KM-1): (A,B,C) and c-Jun (H-79): (D,E,F).
Application
Antibodies tested include p-c-Jun (Ser 63/73)-R: (A,B,C) and c-Jun (H-79): (D,E,F).
Application
Western blot analysis of c-Jun expression in non-transfected CHO: (A), human c-Jun transfected CHO: (B) and NIH/3T3 (C) whole cell lysates.
Application
Western blot analysis of c-Jun expression in non-transfected 293T: (A), mouse c-Jun transfected 293T: (B) and NIH/3T3 (C) whole cell lysates.
Application
Western blot analysis of c-Jun expression in non-transfected 293T: (A), mouse c-Jun transfected 293T: (B) and NIH/3T3 (C) whole cell lysates.
Application
Western blot analysis of c-Jun expression in non-transfected: (A) and mouse c-Jun transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of c-Jun expression in non-transfected: (A) and human c-Jun transfected: (B) 293 whole cell lysates.
Application
Western blot analysis of c-Jun expression in non-transfected (A) and human c-Jun transfected (B) CHO whole cell lysates.
Application
Intracellular FCM analysis of fixed and permeabilized control (green line histogram) and PMA-induced (solid orange histogram) NIH/3T3 cells. Dotted pink histogram represents the isotype control, normal rabbit IgG: .
Application
Antibodies tested include c-Jun (H-79): (A,B), p-c-Jun (Ser 73)-R: (C,D) and p-c-Jun (Ser 63/73)-R: (E,F).
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded normal human prostate tissue (A) and immunofluorescence staining of methanol-fixed NIH/3T3 cells (B) showing nuclear localization.
Application
Western blot analysis of c-Jun p39 expression in untreated (A) and phorbol ester-induced (B) NIH/3T3 nuclear extracts.| Application Notes | recommended for detection of c-Jun p39 of mouse, rat, human and Xenopus origin by WB, IP, IF, IHC(P), FCM and ELISA also reactive with additional species, including canine, bovine and porcine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
