Application
Western blot analysis of PKC β expression in non-transfected: (A) and human PKC β transfected: (B) 293T whole cell lysates.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic staining.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human tonsil tissue showing cytoplasmic staining.Application
PKC antibodies used include PKC α (C-20), PKC βI (C-16), PKC βII (C-18), PKC γ (C-19), PKC ε (C-15), PKC ζ (C-20) and PKC η (C-15).Application
Western blot analysis of PKC βII expression in HeLa (A) and A-431 (B) whole cell lysates.Application
Western blot analysis of PKC βII expression in K-562 whole cell lysate.| Product Name | PKC βII Antibody (C-18) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Members of the protein kinase C (PKC) family play a key regulatory role in a variety of cellular functions including cell growth and differentiation, gene expression, hormone secretion and membrane function. PKCs were originally identified as serine/threonine protein kinases whose activity was dependent on calcium and phospholipids. Diacylglycerols (DAG) and tumor promoting phorbol esters bind to and activate PKC. PKCs can be subdivided into at least two major classes including conventional (c) PKC isoforms (å, ∫I, ∫II and ©) and novel (n) PKC isoforms (∂, é, Ω, ñ and †). Patterns of expression for each PKC isoform differ among tissues and PKC family members exhibit clear differences in their cofactor dependencies. For instance, the kinase activities of nPKC ∂ and é are independent of Ca2+. On the other hand, nPKC ∂ and é, as well as all of the cPKC members, possess phorbol ester-binding activities and kinase activities. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of PKC &betaII of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including bovine and porcine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the C-terminus of PKC &beta II of human origin |
|---|---|
| Tissue Specificity | epitope mapping at the C-terminus of PKC βII of human origin |
| Entrez Gene | 5579 |
|---|
Application
Western blot analysis of PKC β expression in non-transfected: (A) and human PKC β transfected: (B) 293T whole cell lysates.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic staining.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human tonsil tissue showing cytoplasmic staining.
Application
PKC antibodies used include PKC α (C-20), PKC βI (C-16), PKC βII (C-18), PKC γ (C-19), PKC ε (C-15), PKC ζ (C-20) and PKC η (C-15).
Application
Western blot analysis of PKC βII expression in HeLa (A) and A-431 (B) whole cell lysates.
Application
Western blot analysis of PKC βII expression in K-562 whole cell lysate.| Application Notes | recommended for detection of PKC &betaII of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including bovine and porcine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
