Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human small intestine tissue showing cytoplasmic staining of glandular cells.Application
Western blot analysis of ACK expression in non-transfected: (A) and truncated mouse ACK transfected: (B) 293T whole cell lysates.Application
Antibodies tested include ACK (H-172): (A,B) and ACK (A-11): (C,D,E).| Product Name | ACK Antibody (H-172) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | The Ras-related Rho subfamily of GTP-binding proteins (p21s), which includes Rho, Rac and Cdc42Hs, is implicated in different aspects of cytoskeletal organization. These proteins resemble Ras p21 in that their active GTP-bound form is inactivated by intrinsic hydrolysis of the GTP to GDP, which can be stimulated by GTPase-activating proteins (GAPs). ACK, a tyrosine kinase that specifically binds Cdc42Hs in its GTP-bound form, has been described. This binding is mediated by a unique sequence of 47 amino acids C-terminal to an SH3 domain and inhibits both the intrinsic and GAP-stimulated GTPase activity of Cdc42Hs. These findings suggest that ACK may represent a new class of proteins that sustains the GTP-bound active form of the Rho subfamily of GTP binding proteins and which is directly linked to a tyrosine phosphorylation pathway. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of ACK of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including canine, bovine and porcine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 920-1036 mapping at the C-terminus of ACK of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 920-1036 mapping at the C-terminus of ACK of human origin |
| Entrez Gene | 10188 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human small intestine tissue showing cytoplasmic staining of glandular cells.
Application
Western blot analysis of ACK expression in non-transfected: (A) and truncated mouse ACK transfected: (B) 293T whole cell lysates.
Application
Antibodies tested include ACK (H-172): (A,B) and ACK (A-11): (C,D,E).| Application Notes | recommended for detection of ACK of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including canine, bovine and porcine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
