Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human esophagus tissue showing cytoplasmic staining of squamous epithelial cells.Application
Western blot analysis of TGase2 expression in HEL 92.1.7 (A) and HUV-EC-C (B) whole cell lysates.Application
Immunofluorescence staining of normal mouse kidney frozen section showing cytoplasmic and extracellular staining.Application
Immunofluorescence staining of methanol-fixed ECV304 cells showing cytoplasmic localization.| Product Name | TGase2 Antibody (H-237) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Terminally differentiating mammalian epidermal cells acquire an insoluble, 10 to 20 nm thick protein deposit on the intracellular surface of the plasma membrane known as the cross-linked cell envelope (CE). The CE is a component of the epidermis that is generated through formation of disulfide bonds and g-glutamyl-lysine isodipeptide bonds, which are formed by the action of transglutaminases (TGases). TGases are intercellularly localizing, Ca2+-dependent enzymes that catalyze the formation of isopeptide bonds by transferring an amine on to glutaminyl residues, thereby cross-linking glutamine residues and lysine residues in substrate proteins. TGases influence numerous biological processes, including blood coagulation, epidermal differentiation, seminal fluid coagulation, fertilization, cell differentiation and apoptosis. Human keratinocyte transglutaminase (TGase1) is a membrane associated, 817 amino acid protein. Human tissue transglutaminase (TGase2) is an endothelial cell specific, 687 amino acid protein. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of TGase2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | raised against amino acids 451-687 of TGase2 of human origin |
|---|---|
| Tissue Specificity | raised against amino acids 451-687 of TGase2 of human origin |
| Entrez Gene | 7052 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human esophagus tissue showing cytoplasmic staining of squamous epithelial cells.
Application
Western blot analysis of TGase2 expression in HEL 92.1.7 (A) and HUV-EC-C (B) whole cell lysates.
Application
Immunofluorescence staining of normal mouse kidney frozen section showing cytoplasmic and extracellular staining.
Application
Immunofluorescence staining of methanol-fixed ECV304 cells showing cytoplasmic localization.| Application Notes | recommended for detection of TGase2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
