Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human pancreas tissue showing nuclear staining of glandular cells.Application
Western blot analysis of SLUG expression in non-transfected: (A) and human SLUG transfected: (B) 293 whole cell lysates.| Product Name | SLUG Antibody (H-140) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | The SNAIL family of developmental regulatory proteins is a group of widely conserved zinc-finger proteins that regulate transcription and include the mammalian proteins SLUG, SNAI 1 (the human homolog of Drosophila SNAIL) and Smuc. SNAI 1 and SLUG are expressed in placenta and in adult heart, liver, and skeletal muscle. SNAI 1, and the corresponding mouse homolog Sna, each contain three classic zinc fingers and one atypical zinc finger, while SLUG contains five zinc finger regions and a transcriptional repression domain at the amino terminus, which enables SLUG to act as a negative regulator of gene expression. SLUG is implicated in the generation and migration of neural crest cells in human embryos and also contributes to limb bud development. In addition, SLUG also constitutes a cellular anti-apoptotic transcription factor that effectively prevents apoptosis in murine pro-B cells deprived of IL-3. The SNAIL-related gene from murine skeletal muscle cells, Smuc, is highly expressed in skeletal muscle and thymus and can, likewise, repress gene transcription. Smuc preferentially associates with CAGGTG and CACCTG E-box motifs (CANNTG) on DNA and involves the five putative DNA-binding zinc finger domains at the C-terminal region of Smuc. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISAGSChIP |
| recommended for detection of SLUG of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 21-160 mapping near the N-terminus of SLUG of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 21-160 mapping near the N-terminus of SLUG of human origin |
| Entrez Gene | 6591 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human pancreas tissue showing nuclear staining of glandular cells.
Application
Western blot analysis of SLUG expression in non-transfected: (A) and human SLUG transfected: (B) 293 whole cell lysates.| Application Notes | recommended for detection of SLUG of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
