Application
Western blot analysis of LMO4 expression in non-transfected 293T: (A), human LMO4 transfected 293T: (B) and IMR-32 (C) whole cell lysates.Application
Western blot analysis of LMO4 expression in non-transfected 293T: (A), mouse LMO4 transfected 293T: (B) and IMR-32 (C) whole cell lysates.| Product Name | LMO4 Antibody (H-50) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | The LIM-only (LMO) proteins, LMO1 and LMO2, are nuclear factors that are characterized by a conserved LIM domain (1). The LIM domain consists of a cysteine-rich zinc-binding motif that is present in a variety of transcription factors, including the LIM homeobox (LHX) proteins expressed in the central nervous system and involved in cell differentiation (2). LMO1 and LMO2 are expressed in the adult CNS in a cell type-specific manner, where they are differentially regulated by neuronal activity and are involved in regulating the cellular differentiated phenotype of neurons (3). LMO2 lacks a specific DNA-binding homeobox domain but rather assembles into transcriptional regulatory complexes to mediate gene expression by interacting with the widely expressed nuclear LIM interactor (NLI) (4). NLI, known also as CLIM-1, and the related protein CLIM-2 facilitate the formation of heteromeric LIM complexes and also enhance the nuclear retention of LIM proteins (5). LMO2 and the related protein LMO4 are expressed in thymic precursor cells (6). LMO4 is also expressed in mature T cells, cranial neural crest cells, somite, dorsal limb bud mesenchyme, motor neurons, and Schwann cell progenitors (7). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of LMO4 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 116-165 of LMO4 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 116-165 of LMO4 of human origin |
| Entrez Gene | 8543 |
|---|
Application
Western blot analysis of LMO4 expression in non-transfected 293T: (A), human LMO4 transfected 293T: (B) and IMR-32 (C) whole cell lysates.
Application
Western blot analysis of LMO4 expression in non-transfected 293T: (A), mouse LMO4 transfected 293T: (B) and IMR-32 (C) whole cell lysates.| Application Notes | recommended for detection of LMO4 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
