Application
Western blot analysis of Elf-1 expression in non-transfected: (A) and human Elf-1 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of Elf-1 expression in 293T (A) and HeLa (B) whole cell lysates.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human duodenum tissue showing nuclear staining of glandular cells. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.Application
Western blot analysis of Elf-1 expression in HeLa (A), A-431 (B), A-673 (C), K-562 (D) and Jurkat (E) nuclear extracts.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human colon carcinoma tissue showing intense nuclear staining.| Product Name | Elf-1 Antibody (C-20) HRP |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping at the C-terminus of Elf-1 of human origin |
| Modification Notes | Ets-1 is the prototype member of a family of genes identified on the basis of homology to the v-Ets oncogene isolated from the E26 erythroblastosis virus. This family of genes currently includes Ets-1, Ets-2, Erg-1–3, Elk-1, Elf-1, Elf-5, NERF, PU.1, PEA3, ERM, FEV, ER8l, Fli-1, TEL, Spi-B, ESE-1, ESE-3A, Net, ABT1 and ERF. Members of the Ets gene family exhibit varied patterns of tissue expression, and share a highly conserved carboxy terminal domain containing a sequence related to the SV40 large T antigen nuclear localization signal sequence. This conserved domain is essential for Ets-1 binding to DNA and is likely to be responsible for the DNA-binding activity of all members of the Ets gene family. Elf-1 is a lymphoid-specific member of the Ets family that has been shown to regulate inducible gene expression during T cell activation. Elf-1 contains a sequence motif that is highly related to the Rb-binding sites common to several viral oncoproteins and binds to the pocket region of Rb both in vivo and in vitro. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISAGSChIP |
| recommended for detection of Elf-1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine and canine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the C-terminus of Elf-1 of human origin |
|---|---|
| Tissue Specificity | epitope mapping at the C-terminus of Elf-1 of human origin |
| Entrez Gene | 1997 |
|---|
Application
Western blot analysis of Elf-1 expression in non-transfected: (A) and human Elf-1 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of Elf-1 expression in 293T (A) and HeLa (B) whole cell lysates.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human duodenum tissue showing nuclear staining of glandular cells. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.
Application
Western blot analysis of Elf-1 expression in HeLa (A), A-431 (B), A-673 (C), K-562 (D) and Jurkat (E) nuclear extracts.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human colon carcinoma tissue showing intense nuclear staining.| Application Notes | recommended for detection of Elf-1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine and canine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
