Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human colon tissue showing cytoplasmic and nuclear staining of glandular cells.Application
Antibodies tested include NFκB p50 (C-19): , NFκB p50 (E-10): , NFκB p50 (H-119): , NFκB p65 (C-20): , NFκB p65 (A): , NFκB p65 (H-286): , HDAC3 (H-99): , HDAC3 (N-19): , Bcl-3 (C-14): , Bcl-3 (H-146): . Data kindly provided by M.G. Rosenfeld and reproduced with permission from Baek et al., Cell 2002, 110: 55-67.Application
Western blot analysis of Bcl-3 expression in NAMALWA (A) and WEHI-3 (B) whole cell lysates.| Product Name | Bcl-3 Antibody (C-14) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | On the basis of both functional and structural considerations, members of the IkB family of proteins can be divided into three groups. The first of these groups, IkB-a, includes the avian protein pp40 and the mammalian Mad 3, both of which inhibit binding of p50-p65 NFkB complex or Rel protein to their cognate binding sites but do not inhibit the binding of p50 homodimer to kB sites, suggesting that the IkB-a family binds to the p65 subunit of p50-p65 heterocomplex through ankyrin repeats. The second member of the IkB family is represented by a protein designated IkB-b. The third group of IkB proteins is represented by IkB-g, a protein identical in sequence with the C-terminal domain of the p110 precursor of NFkB p50 and expressed predominantly in lymphoid cells. The proto-oncogene Bcl-3, believed to be involved in certain human B cell leukemias, encodes a protein that functions as an IkB-like molecule for native NFkB but is specific for the p50 subunit. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISAChIP |
| recommended for detection of Bcl-3 of mouse, rat and human origin by WB, IP, IF, ihc(P) and ELISA | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the C-terminus of Bcl-3 of human origin |
|---|---|
| Tissue Specificity | epitope mapping at the C-terminus of Bcl-3 of human origin |
| Entrez Gene | 602 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human colon tissue showing cytoplasmic and nuclear staining of glandular cells.
Application
Antibodies tested include NFκB p50 (C-19): , NFκB p50 (E-10): , NFκB p50 (H-119): , NFκB p65 (C-20): , NFκB p65 (A): , NFκB p65 (H-286): , HDAC3 (H-99): , HDAC3 (N-19): , Bcl-3 (C-14): , Bcl-3 (H-146): . Data kindly provided by M.G. Rosenfeld and reproduced with permission from Baek et al., Cell 2002, 110: 55-67.
Application
Western blot analysis of Bcl-3 expression in NAMALWA (A) and WEHI-3 (B) whole cell lysates.| Application Notes | recommended for detection of Bcl-3 of mouse, rat and human origin by WB, IP, IF, ihc(P) and ELISA |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
