Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nucleolar and nuclear localization.Application
Western blot analysis of CREM expression in Jurkat nuclear extract.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human testis tissue showing nuclear staining of cells in ductus seminiferus at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Western blot analysis of ATF-1/CREB gene family product expression in Jurkat (A) and KNRK (B) nuclear extracts and recombinant mouse His-tagged CREM (FL): (C) and an unrelated His-tagged protein (D).| Product Name | CREM Antibody (X-12) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Eukaryotic gene transcription is regulated by sequence specific transcription factors which bind modular cis acting promoter and enhancer elements. The cAMP response element (CRE), one of the best studied of such elements, consists of the palindromic octanucleotide TGACGTCA. Several CRE binding proteins have been identified within the ATF/CREB family, the best characterized of which include CREB-1, CREB-2 (also designated ATF-4), ATF-1, ATF-2 and ATF-3. These proteins share highly related COOH terminal leucine zipper dimerization and basic DNA binding domains but are highly divergent in their amino terminal domains. Members of the CREB-related CREM-å and CREM-∫ proteins share a kinase-inducible domain with CREB but act as transcriptional repressors rather than activators. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISAGSChIP |
| recommended for detection of CREM of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA may cross-react with other ATF/CREB proteins and other CREM isoforms also reactive with additional species, including porcine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | produced by immunization with full length polyhistidine tagged CREM fusion protein of mouse origin |
|---|---|
| Tissue Specificity | produced by immunization with full length polyhistidine tagged CREM fusion protein of mouse origin |
| Entrez Gene | 1390 |
|---|
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nucleolar and nuclear localization.
Application
Western blot analysis of CREM expression in Jurkat nuclear extract.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human testis tissue showing nuclear staining of cells in ductus seminiferus at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Western blot analysis of ATF-1/CREB gene family product expression in Jurkat (A) and KNRK (B) nuclear extracts and recombinant mouse His-tagged CREM (FL): (C) and an unrelated His-tagged protein (D).| Application Notes | recommended for detection of CREM of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA may cross-react with other ATF/CREB proteins and other CREM isoforms also reactive with additional species, including porcine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
