Application
Western blot analysis of CENP-C expression in HeLa whole cell lysate.Application
Immuno?fluorescence staining of methanol-fixed HeLa cells showing nuclear local?ization.| Product Name | CENP-C Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | A replicated chromosome includes two kinetochores that control chromosome segregation during mitosis. The evolutionarily conserved Centromere Protein-C, CENP-C, is a kinetochore assembly protein. CENP-C is located on the fibers of the kinetochore and constitutes a kinetochore organizing center that tightly associates with DNA. CENP-C is necessary for the formation of a functional centromere, which indicates that CENP-C is important for mitotic progression. In addition, CENP-C is lost from centromeres during herpes simplex virus 1 infection, causing substantial structural changes in the kinetochore, which suggests that the structure of CENP-C is regulated during the cell cycle. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of CENP-C of human and, to a lesser extent, mouse and rat origin by WB, IP, IF and ELISA | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 644-943 mapping at the C-terminus of CENP-C of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 644-943 mapping at the C-terminus of CENP-C of human origin |
| Entrez Gene | 1060 |
|---|
Application
Western blot analysis of CENP-C expression in HeLa whole cell lysate.
Application
Immuno?fluorescence staining of methanol-fixed HeLa cells showing nuclear local?ization.| Application Notes | recommended for detection of CENP-C of human and, to a lesser extent, mouse and rat origin by WB, IP, IF and ELISA |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
