Application
Western blot analysis of EB1 expression in non-transfected 293T: (A), human EB1 transfected 293T: (B) and SW480 (C) whole cell lysates.Application
Western blot analysis of EB1 expression in non-transfected: (A) and human EB1 transfected: (B) 293T whole cell lysates.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human urinary bladder tissue showing cytoplasmic staining of surface epithelial cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.Application
Western blot analysis of EB1 expression in HeLa (A) and LS1034 (B) whole cell lysates.| Product Name | EB1 Antibody (H-70) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | EB1 (MAPRE2, microtubule-associated protein, RP/EB family, member 2, EB2, RP1) may influence tumorigenesis of colorectal cancers and proliferative control of normal cells. EB1 belongs to the intermediate/early gene family, involved in the signal transduction cascade downstream of the T cell receptor (TRC). Colorectal cancer is caused by the pathologic transformation of normal colonic epithelium to an adenomatous polyp, which can become an invasive cancer. APC (adenomatous polyposis coli) is a tumor suppressor gene, the mutation of which is one of the earliest events in colorectal carcinogenesis. A majority of the mutations result in the loss of the carboxy terminus of APC. EB1 has been shown to bind to the carboxy terminal region of APC, which implicates EB1 in APC suppression of colonic cancer. EB1 overexpression may play a role in the development of human esophageal squamous cell carcinoma by affecting APC function and activating the ∫-catenin/TCF pathway. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of EB1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 133-202 of EB1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 133-202 of EB1 of human origin |
| Entrez Gene | 22919 |
|---|
Application
Western blot analysis of EB1 expression in non-transfected 293T: (A), human EB1 transfected 293T: (B) and SW480 (C) whole cell lysates.
Application
Western blot analysis of EB1 expression in non-transfected: (A) and human EB1 transfected: (B) 293T whole cell lysates.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human urinary bladder tissue showing cytoplasmic staining of surface epithelial cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.
Application
Western blot analysis of EB1 expression in HeLa (A) and LS1034 (B) whole cell lysates.| Application Notes | recommended for detection of EB1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
