Application
Western blot analysis of MSH3 expression in MOLT-4 (A) and HeLa (B) whole cell lysates.Application
Western blot analysis of MSH3 expression in 293T whole cell lysate (A) and HeLa nuclear extract (B).Application
Western blot analysis of MSH3 expression in SW480 whole cell lysate.Application
Western blot analysis of MSH3 expression in non-transfected control (A) and MSH3 siRNA transfected (B) HeLa cells. Blot probed with MSH3 (H-300): . α-actinin (H-2): used as specificity and loading control.| Product Name | MSH3 Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | DNA mismatch repair (MMR) is essential for maintaining the integrity of the genome during replication. This process is highly conserved across bacterial and eukaryotic systems, as many of the genes expressed in bacteria are closely related to the yeast and mammalian homologs. In bacteria two proteins, MutS and MutL, form homodimeric complexes that are responsible for recognizing and facilitating MMR. Human homologs of these proteins include MSH2 and MSH3 (MutS homolog 2 and 3), and the corresponding human homologs of MutL are MLH1, PMS1, PMS2 and MLH3. MSH2 and MSH3 form heterodimers that cooperatively mediate MMR. MLH3 preferentially dimerizes with MLH1 to repair DNA mismatches and restore the stability to the genome. Mutations in the genes encoding MSH2 and MLH1 induce microsatellite instablitiy of the DNA. These mutations are associated with the occurrence of hereditary nonpolyposis colorectal cancer (HNPCC) and are a common feature in the progression of many other cancers. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of MSH3 of mouse, rat and human origin by WB, IP, IF and ELISA | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 61-360 of MSH3 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 61-360 of MSH3 of human origin |
| Entrez Gene | 4437 |
|---|
Application
Western blot analysis of MSH3 expression in MOLT-4 (A) and HeLa (B) whole cell lysates.
Application
Western blot analysis of MSH3 expression in 293T whole cell lysate (A) and HeLa nuclear extract (B).
Application
Western blot analysis of MSH3 expression in SW480 whole cell lysate.
Application
Western blot analysis of MSH3 expression in non-transfected control (A) and MSH3 siRNA transfected (B) HeLa cells. Blot probed with MSH3 (H-300): . α-actinin (H-2): used as specificity and loading control.| Application Notes | recommended for detection of MSH3 of mouse, rat and human origin by WB, IP, IF and ELISA |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
