Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human kidney tissue showing cytoplasmic staining of cells in tubules.Application
Immunofluorescence staining of methanol-fixed HeLa cells cytoplasmic staining.Application
Western blot analysis of Bad expression in whole cell lysates prepared from control (A) and serum-starved (B) NIH/3T3 cells.| Product Name | Bad Antibody (K-17) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | The Bcl-2 family of proteins is characterized by its ability to modulate cell death (apoptosis) under a broad range of physiologic conditions. Bcl-2 and several related proteins function to inhibit apoptosis (1,2), while other members of the Bcl-2 family such as Bax (3,4) and Bak (5,6) enhance cell death under various conditions. For instance, Bcl-xL represses cell death, while its shorter form, Bcl-xS, promotes apoptosis (7). A protein designated Bad exhibits homology to Bcl-2, limited to the BH1 and BH2 domains (8). Bad functions to dimerize with Bcl-xL and with Bcl-2, but not with Bax, Bcl-xS, Mcl-1, A1 or itself. In mammalian cells, Bad binds with greater affinity to Bcl-xL than to Bcl-2, and reverses the death repressor activity of Bcl-xL but not Bcl-2 (8). Dimerization of Bad with Bcl-xL results in displacement of Bax from Bcl-xL:Bax complexes, thereby causing restoration of Bax-mediated apoptosis (8). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of Bad of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping near the N-terminus of Bad of mouse origin |
|---|---|
| Tissue Specificity | epitope mapping near the N-terminus of Bad of mouse origin |
| Entrez Gene | 572 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human kidney tissue showing cytoplasmic staining of cells in tubules.
Application
Immunofluorescence staining of methanol-fixed HeLa cells cytoplasmic staining.
Application
Western blot analysis of Bad expression in whole cell lysates prepared from control (A) and serum-starved (B) NIH/3T3 cells.| Application Notes | recommended for detection of Bad of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
