Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human appendix tissue showing nuclear staining of inflammatory cells.Application
Western blot analysis of CoREST expression in MOLT-4 nuclear extract.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.| Product Name | CoREST Antibody (H-65) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | NRSF (neuron-restrictive silencer factor, also designated XBR and REST for RE1-silencing factor) is a silencer protein that represses neuronal gene transcription in non-neuronal cells. NRSF-mediated repression requires histone deacetylase activity because repressed genes are associated with hypoacetylated chromatin. HDAC is recruited to the NRSF repressor complex by two co-repressors, Sin3A and CoREST. CoREST interacts with a single zinc finger motif in the carboxy-terminal repressor domain of NRSF, whereas Sin3A interacts with NRSF’s amino-terminal repressor domain. In addition, CoREST interacts with HDAC through a SANT domain, which is found in other HDAC interacting proteins such as NCoR, MTA1 and MTA2. CoREST is an integral component of the NRSF repressor complex. Its functionality has been conserved in several species, including human, mouse, Xenopus, and C. elegans. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of CoREST of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 246-310 mapping within an internal region of CoREST of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 246-310 mapping within an internal region of CoREST of human origin |
| Entrez Gene | 23186 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human appendix tissue showing nuclear staining of inflammatory cells.
Application
Western blot analysis of CoREST expression in MOLT-4 nuclear extract.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.| Application Notes | recommended for detection of CoREST of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
