Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human spleen tissue showing cytoplasmic staining of subset of cells in red pulp.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human spleen tissue showing cytoplasmic and extracellular staining of cells in red pulp at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Western blot analysis of human recombinant Nidogen.Application
Immunofluorescence staining of normal human cornea frozen section showing basement membrane and anterior corneal epithelium staining.| Product Name | Nidogen Antibody (H-200) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Basement membranes are the earliest extracellular matrices produced during embryogenesis. They are synthesized and incorporated into the supramolecular architecture of several components, including laminins, Collagen IV, Nidogen and proteoglycans. Nidogen/Entactin, a sulfated glycoprotein, acts as a link between the extracellular matrix molecules Laminin 1 and Collagen Type IV, and thereby participates in the assembly of basement membranes. Nidogen is a highly conserved member of the Nidogen family, which also includes Nidogen-2. Nidogen-2 has a high level of N- and O-glycosylation, and it interacts with Collagens Type I and IV and Perlecan at a comparable level to Nidogen. Nidogen is synthesized and secreted in primary and established mesenchymal peritubular cells and myoepithelial cells, and it affects adhesion of peritubular cells in an autocrine manner. Nidogen is expressed during embryonic and fetal development exclusively in fully developed basement membranes of the ectoderm and is not expressed in the developing endodermal basement membrane or in membranes disrupted during mesoderm formation. Nidogen also cooperates with Laminin 1 to regulate b-casein expression. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of Nidogen of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 201-400 mapping within an internal region of Nidogen of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 201-400 mapping within an internal region of Nidogen of human origin |
| Entrez Gene | 4811 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human spleen tissue showing cytoplasmic staining of subset of cells in red pulp.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human spleen tissue showing cytoplasmic and extracellular staining of cells in red pulp at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Western blot analysis of human recombinant Nidogen.
Application
Immunofluorescence staining of normal human cornea frozen section showing basement membrane and anterior corneal epithelium staining.| Application Notes | recommended for detection of Nidogen of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
