Application
Western blot analysis of ε-sarcoglycan expression in A549 whole cell lysate (A) and mouse heart tissue extract (B).| Product Name | ε-sarcoglycan Antibody (H-67) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | The sarcoglycan transmembrane proteins are members of the dystrophin complex (1). Sarcoglycans cluster together to form a complex, which is localized in the cell membrane of skeletal, cardiac, and smooth muscle fibers (1). Four sarcoglycan subunit proteins, designated å-, ∫-, ©- and ∂-sarcoglycan, form a complex on the skeletal muscle cell surface membrane (2). A genetic defect in any one of these proteins causes the loss or marked decrease of the whole sarcoglycan complex, which is observed in the autosomal recessive muscular dystrophy, sarcoglycanopathy (2-6). In smooth muscle, ∫- and ∂-sarcoglycans are associated with é-sarcoglycan, a glycoprotein homologous to å-sarcoglycan (5). Additionally, a complete deficiency in ∂-sarcoglycan is the cause of the Syrian hamster BIO.14 cardiomyopathy (3). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of &epsilon-sarcoglycan of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 24-90 mapping within an extracellular domain of &epsilon -sarcoglycan of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 24-90 mapping within an extracellular domain of ε-sarcoglycan of human origin |
| Entrez Gene | 8910 |
|---|
Application
Western blot analysis of ε-sarcoglycan expression in A549 whole cell lysate (A) and mouse heart tissue extract (B).| Application Notes | recommended for detection of &epsilon-sarcoglycan of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
