Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human stomach tissue showing cytoplasmic and nuclear staining of glandular and interstitial cells.Application
Western blot analysis of FRAP expression in 293T (A), HeLa (B), Jurkat (C), MOLT-4 (D) and K-562 (E) whole cell lysates.Application
IImmunofluorescence staining of normal mouse liver frozen section showing cytoplasmic and nuclear staining.Application
Western blot analysis of FRAP expression in Calyculin A-treated HeLa whole cell lysate.| Product Name | mTOR Antibody (H-266) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | The phosphatidylinositol kinase (PIK) family members fall into two distinct subgroups. The first subgroup contains proteins such as the PI 3- and PI 4-kinases and the second group comprises the PIK-related kinases. The PIK-related kinases include Atm, DNA-PKCS and FRAP. These proteins have in common a region of homology at their carboxy-termini that is not present in the PI 3- and PI 4-kinases. The Atm gene is mutated in the autosomal recessive disorder ataxia telangiectasia (AT) that is characterized by cerebellar degeneration (ataxia) and the appearance of dilated blood vessels (telangiectases) in the conjunctivae of the eyes. AT cells are hypersensitive to ionizing radiation, impaired in mediating the inhibition of DNA synthesis and display delays in p53 induction. DNA-PK is a heterotrimeric DNA binding enzyme that is composed of a large subunit, DNA-PKCS, and two smaller subunits collectively known as Ku. The loss of DNA-PK leads to defects in DSB repair and V(D)J recombination. FRAP can autophosphorylate on serine and bind to Rapamycin/FKBP. FRAP is also an upstream regulator of S6 kinase and has been implicated in the regulation of p27 and p21 expression. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of mTOR of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1920-2185 of mTOR of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1920-2185 of mTOR of human origin |
| Entrez Gene | 2475 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human stomach tissue showing cytoplasmic and nuclear staining of glandular and interstitial cells.
Application
Western blot analysis of FRAP expression in 293T (A), HeLa (B), Jurkat (C), MOLT-4 (D) and K-562 (E) whole cell lysates.
Application
IImmunofluorescence staining of normal mouse liver frozen section showing cytoplasmic and nuclear staining.
Application
Western blot analysis of FRAP expression in Calyculin A-treated HeLa whole cell lysate.| Application Notes | recommended for detection of mTOR of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
