Application
Western blot analysis of Adducin γ expression in NIH/3T3 whole cell lysate.Application
Western blot analysis of Adducin γ expression in non-transfected: (A) and mouse Adducin γ transfected: (B) 293T whole cell lysates.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human gall bladder tissue showing membrane staining of glandular cells (low and high magni?fication). Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Immunofluorescence staining of methanol-fixed JEG-3 cells showing cytoplasmic localization.Application
Western blot analysis of Adducin γ expression in F9 whole cell lysate.| Product Name | Adducin γ Antibody (H-60) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Adducins are a family of cytoskeleton proteins encoded by three genes (å, ∫, ©). Adducin is a protein associated with the inner leaflet of the plasma membrane and is one of the proteins localized at the spectrin-actin junction of the membrane skeleton. The cortical actin cytoskeletal network is lost during apoptosis and Adducins are central in the cortical actin network organization. Adducin å is a cytoskeletal protein involved with sodium-pump activity in the renal tubule and is associated with hypertension. The expression of Adducin å and Adducin © is ubiquitous in contrast to the restricted expression of Adducin ∫. Adducin ∫ is expressed at high levels in brain and hematopoietic tissues, such as bone marrow, in humans, and in spleen in mice. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of Adducin &gamma of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine and porcine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 571-630 mapping near the C-terminus of Adducin &gamma of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 571-630 mapping near the C-terminus of Adducin γ of human origin |
| Entrez Gene | 120 |
|---|
Application
Western blot analysis of Adducin γ expression in NIH/3T3 whole cell lysate.
Application
Western blot analysis of Adducin γ expression in non-transfected: (A) and mouse Adducin γ transfected: (B) 293T whole cell lysates.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human gall bladder tissue showing membrane staining of glandular cells (low and high magni?fication). Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Immunofluorescence staining of methanol-fixed JEG-3 cells showing cytoplasmic localization.
Application
Western blot analysis of Adducin γ expression in F9 whole cell lysate.| Application Notes | recommended for detection of Adducin &gamma of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine and porcine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
