Application
Western blot analysis of claudin-11 expression in mouse brain tissue extract.Application
Western blot analysis of claudin-11 expression in non-transfected: (A) and human claudin-11 transfected: (B) 293 whole cell lysates.Application
Immunofluorescence staining of normal mouse skin frozen section showing membrane staining.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human cerebral cortex tissue showing membrane staining of neuronal and non-neuronal cells (low and high magni?fication). Kindly provided by The Swedish Human Protein Atlas (HPA) program.| Product Name | claudin-11 Antibody (H-107) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | The claudin superfamily consists of many structurally related proteins in humans. These proteins, which include claudin-1 through -18, are important structural and functional components of tight junctions in paracellular transport. Claudins are located in both epithelial and endothelial cells in all tight junction-bearing tissues. Three classes of proteins are known to localize to tight junctions, including the claudins, Occludin and junction adhesion molecule (JAM). Claudins, which consist of four transmembrane domains and two extracellular loops, make up tight junction strands. Claudin expression is often highly restricted to specfic regions of different tissues and may have an important role in transcellular transport through tight junctions. Claudin-11 is an oligodendrocyte-specific protein that is expresed in the tight junctions of Sertoli cells and myelin sheaths in mice. In addition, claudin-11 is expressed in the epithelial tight junctions of the choroid plexus. The human claudin-11 gene maps to chromosome 3q26.2-q26.3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of claudin-11 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 101-207 mapping at the C-terminus of claudin-11 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 101-207 mapping at the C-terminus of claudin-11 of human origin |
| Entrez Gene | 5010 |
|---|
Application
Western blot analysis of claudin-11 expression in mouse brain tissue extract.
Application
Western blot analysis of claudin-11 expression in non-transfected: (A) and human claudin-11 transfected: (B) 293 whole cell lysates.
Application
Immunofluorescence staining of normal mouse skin frozen section showing membrane staining.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human cerebral cortex tissue showing membrane staining of neuronal and non-neuronal cells (low and high magni?fication). Kindly provided by The Swedish Human Protein Atlas (HPA) program.| Application Notes | recommended for detection of claudin-11 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
