Application
Western blot analysis of paxillin phosphorylation in untreated (A) and lambda protein phosphatase treated (B) A-431 whole cell lysates.Application
p-paxillin (Tyr 31)-R: .Western blot analysis of paxillin phosphorylation in untreated (A), pervanadate treated (B) and pervanadate and lambda protein phosphatase (A) treated (C) A-431 whole cell lysates.Application
Immunofluorescence staining of methanol-fixed serum-starved, then serum-treated HeLa cells showing membrane and nuclear localization.Application
Intracellular FCM analysis of fixed and permeabilized control (green line histogram) and serum-starved, then serum-treated (solid orange histogram) HeLa cells. Dotted pink histogram represents the isotype control, normal goat IgG: .Application
Western blot analysis of p-paxillin expression in HeLa (A) and serum-treated HeLa (B) following serum starvation.| Product Name | p-paxillin Antibody (Tyr 31) PE |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | The effects of some oncogenes, growth factors and neuropeptides are mediated by tyrosine phosphorylation of focal adhesion kinase (FAK) and paxillin cytoskeletal proteins. A rapid increase in tyrosine phosphorylation of paxillin, FAK and Crk-associated substrate (CAS) are prominent early events triggered by many G-protein-coupled receptors. In addition to G protein-coupled receptors, angiotensin IV (Ang IV), protein kinase C and other proteins can also mediate the tyrosine phosphorylation of paxillin. Paxillin must bind FAK for maximal phosphorylation in response to cell adhesion. FAK may function to direct tyrosine phosphorylation of paxillin in the process of transformation by the src oncogene. Tyrosine phosphorylated FAK and paxillin function to regulate the signaling mechanism of the rapid nongenomic action of dexamethasone on the Actin cytoskeleton. In glomerular epithelial cells, TNFa induces substantial reorganization of Actin cytoskeleton and focal adhesions. TNFa also simultaneoulsy mediates tyrosine phosphorylation of paxillin and FAK, which regulate Actin polymerization and the formation of focal adhesions, and may be directly involved in the redistribution of Actin. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFFCELISA |
| blocking peptide, sc-14035 P also reactive with additional species, including equine, canine and avian | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of paxillin of human origin phosphorylated at Tyr 31 by WB IP IF FCM and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of paxillin of human origin phosphorylated at Tyr 31 by WB, IP, IF, FCM and ELISA |
Application
Western blot analysis of paxillin phosphorylation in untreated (A) and lambda protein phosphatase treated (B) A-431 whole cell lysates.
Application
p-paxillin (Tyr 31)-R: .Western blot analysis of paxillin phosphorylation in untreated (A), pervanadate treated (B) and pervanadate and lambda protein phosphatase (A) treated (C) A-431 whole cell lysates.
Application
Immunofluorescence staining of methanol-fixed serum-starved, then serum-treated HeLa cells showing membrane and nuclear localization.
Application
Intracellular FCM analysis of fixed and permeabilized control (green line histogram) and serum-starved, then serum-treated (solid orange histogram) HeLa cells. Dotted pink histogram represents the isotype control, normal goat IgG: .
Application
Western blot analysis of p-paxillin expression in HeLa (A) and serum-treated HeLa (B) following serum starvation.| Application Notes | blocking peptide, sc-14035 P also reactive with additional species, including equine, canine and avian |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
