Application
Western blot analysis of Rad GTPase expression in NCI-H1688 whole cell lysate.| Product Name | Rad GTPase Antibody (H-143) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | The Ras-encoded family of proteins bind GDP and GTP with high affinity. They possess a low level of intrinsic GTPase activity that increases more than 100-fold when interacting with cytosolic GTPase activating protein (GAP). Ras family members include H-Ras, K-Ras, N-Ras, M-Ras, R-Ras, E-Ras, Rheb, TC 21, RASL11B, and Rad (Ras associated with diabetes) GTPase. Rad GTPase is a GTP-binding protein that is similar to Ras but has unique features. Unlike other small GTPases, Rad GTPase lacks typical prenylation motifs at its C terminus. The Rad GTPase enzyme binds calmodulin, inhibits vascular lesion formation, has low intrinsic GTPase activity and cannot be stimulated by any known GAP molecules. Rad GTPase is expressed in skeletal muscle, cardiac muscle and lung tissues and is overexpressed in the skeletal muscle tissue of individuals with type II diabetes. It is also expressed to a lesser extent in the placenta, adipose tissue and kidney. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Rad GTPase of mouse, rat and human origin by WB, IP, IF and ELISA | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-143 mapping at the N-terminus of Rad GTPase of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-143 mapping at the N-terminus of Rad GTPase of human origin |
| Entrez Gene | 6236 |
|---|
Application
Western blot analysis of Rad GTPase expression in NCI-H1688 whole cell lysate.| Application Notes | recommended for detection of Rad GTPase of mouse, rat and human origin by WB, IP, IF and ELISA |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
