Application
Immunofluorescence staining of formalin-fixed Hep G2 cells showing cytoplasmic localization.Application
Western blot analysis of AFP expression in 293T (A) and HeLa (B) whole cell lysates.Application
Western blot analysis of AFP expression in non-transfected 293T: (A), human AFP transfected 293T: (B) and Hep G2 (C) whole cell lysates.Application
Western blot analysis of human recombinant AFP.| Product Name | AFP Antibody (H-140) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | å-fetoprotein (AFP) is expressed in fetal liver at varying levels throughout development and is present only in trace amounts in normal adult tissues. AFP can be detected at abnormally high concentrations in hepatocellular carcinomas as well as in the plasma and ascitic fluid of adults with hepatoma. High AFP concentrations have been correlated with tumor cell growth, indicating that AFP can serve as a tumor marker. AFP binds copper, nickel and fatty acids, and in some cases may bind serum albumin or estrogen. It has been demonstrated that the AFP promoter is a target for NF-1 (nuclear factor-1), HNF-1 (hepatocyte nuclear factor-1) and C/EBP transcription factors. While HNF-1 binding to the AFP promoter results in AFP expression, NF-1 binding results in a decrease in AFP promoter activity. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of alpha fetoprotein of human and, to a lesser extent, mouse and rat origin by WB, IP, IF and ELISA | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 171-310 mapping to an internal region of AFP (alpha fetoprotein) of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 171-310 mapping to an internal region of AFP (alpha fetoprotein) of human origin |
| Entrez Gene | 174 |
|---|
Application
Immunofluorescence staining of formalin-fixed Hep G2 cells showing cytoplasmic localization.
Application
Western blot analysis of AFP expression in 293T (A) and HeLa (B) whole cell lysates.
Application
Western blot analysis of AFP expression in non-transfected 293T: (A), human AFP transfected 293T: (B) and Hep G2 (C) whole cell lysates.
Application
Western blot analysis of human recombinant AFP.| Application Notes | recommended for detection of alpha fetoprotein of human and, to a lesser extent, mouse and rat origin by WB, IP, IF and ELISA |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
