Application
Western blot analysis of PORCN expression in K-562 (A) and PC-12 (B) whole cell lysates.| Product Name | PORCN Antibody (K-13) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | PORCN (Probable protein-cysteine N-palmitoyltransferase porcupine), also known as MG61, PORC, PPN, por, DHOF or FODH, is a 461 amino acid multi-pass membrane protein that localizes to the endoplasmic reticulum. Expressed as four isoforms that are found in a variety of tissues, including brain, testis, heart, skeletal muscle, spinal cord and lung, PORCN functions to modulate the processing of Wnt proteins. Specifically, PORCN interacts with Wnt-1, Wnt-3, Wnt-4 and Wnt-6 and acts as a protein-cysteine N-palmitoyltransferase that palmitoylates target Wnt proteins. Defects in the gene encoding PORCN are associated with focal dermal hypoplasia (FDH) (also known as Goltz syndrome), an X-linked disorder characterized by improper ectodermal and mesodermal tissue development. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of PORCN of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping within a cytoplasmic domain of PORCN of human origin |
|---|---|
| Tissue Specificity | epitope mapping within a cytoplasmic domain of PORCN of human origin |
| Entrez Gene | 64840 |
|---|
Application
Western blot analysis of PORCN expression in K-562 (A) and PC-12 (B) whole cell lysates.| Application Notes | recommended for detection of PORCN of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
