Application
Western blot analysis of paxillin expression in non-transfected: (A) and human paxillin transfected: (B) 293T whole cell lysates.Application
Antibodies tested include paxillin (H-114): (A,B), p-paxillin (Tyr 181): (C,D) and p-paxillin (Tyr 181)-R: (E,F).Application
Western blot analysis of paxillin expression in CCD-1064Sk (A) and HISM (B) whole cell lysates.| Product Name | paxillin Antibody (H-114) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Paxillin is a focal adhesion phosphoprotein that is localized to the cytoskeleton. Phosphorylation of paxillin has been shown to occur in response to PDGF treatment, v-src transformation or cross-linking of integrins. FAK (focal adhesion kinase) and PYK2 have been shown to phosphorylate paxillin. FAK phosphorylates paxillin specifically on Tyr-118 in vitro. However, FAK phosphorylation does not seem to be required for the recruitment of paxillin to cell adhesion sites. Paxillin may play a role in signal transduction, regulation of cell morphology and the recruitment of structural and signaling molecules to focal adhesions. It has been shown that the amount of paxillin is reduced in mitotic cells by proteolytic downregulation and that paxillin is alternatively phosphorylated on serine rather than on tyrosine and serine during mitosis. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of &alpha, &beta and &gamma isoforms of paxillin of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine and bovine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 155-268 mapping within an internal region of paxillin of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 155-268 mapping within an internal region of paxillin of human origin |
| Entrez Gene | 5829 |
|---|
Application
Western blot analysis of paxillin expression in non-transfected: (A) and human paxillin transfected: (B) 293T whole cell lysates.
Application
Antibodies tested include paxillin (H-114): (A,B), p-paxillin (Tyr 181): (C,D) and p-paxillin (Tyr 181)-R: (E,F).
Application
Western blot analysis of paxillin expression in CCD-1064Sk (A) and HISM (B) whole cell lysates.| Application Notes | recommended for detection of &alpha, &beta and &gamma isoforms of paxillin of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine and bovine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
