Application
Western blot analysis of mouse recombinant ATPIF1 fusion protein.| Product Name | ATPIF1 Antibody (M-78) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Mitochondrial ATP synthases (ATPases) transduce the energy contained in membrane electrochemical proton gradients into the energy required for synthesis of high-energy phosphate bonds. ATPases contain two linked complexes: F1, the hydrophilic catalytic core; and F0, the membrane-embedded protein channel. F1 consists of three å chains and three ∫ chains, which are weakly homologous, as well as one © chain, one ∂ chain and one é chain. F0 consists of three subunits: a, b and c. A mitochondrial F1-ATPase inhibitor protein, ATPIF1 (ATPase inhibitory factor 1), also known as IP, IF1, ATPI or ATPIP (ATPase inhibitor protein), binds to the C-terminal region of a ∫ subunit of the F1-ATPase at low pH values and, via interference of the ∫ and © subunit interaction, ATPIF1 regulates the activity of the F1F0-ATPase. This reversible ATPIF1 binding to F1F0-ATPase also occurs on the surface of endothelial cells. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of ATPIF1 of mouse and rat origin by WB, IP, IF and ELISA | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 27-104 mapping within an internal region of ATPIF1 of mouse origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 27-104 mapping within an internal region of ATPIF1 of mouse origin |
Application
Western blot analysis of mouse recombinant ATPIF1 fusion protein.| Application Notes | recommended for detection of ATPIF1 of mouse and rat origin by WB, IP, IF and ELISA |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
