Application
Western blot analysis of Vav2 expression in HeLa (A) and A-431 (B) whole cell lysates and mouse brain tissue extract (C).Application
Immunofluor?escence staining of normal mouse intestine frozen section showing cytoplasmic staining.Application
Western blot analysis of Vav2 expression in HeLa whole cell lysate.| Product Name | Vav2 Antibody (H-200) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | The Vav gene was originally identified on the basis of its oncogenic activation during the course of gene transfer assays. The major translational product of the Vav proto-oncogene has been identified as a protein containing an array of structural motifs. This protein, known as Vav, Vav1 or p95Vav, contains an N-terminal helix-loop-helix domain and a leucine zipper motif similar to that of Myc family proteins that, if deleted, causes oncogenic activation. In addition, Vav contains an SH2 domain, which could indicate its role as a substrate for tyrosine kinases. Expression of Vav is limited exclusively to cells of hematopoietic origin, including those of the erythroid, lymphoid and myeloid lineages. These results suggest that Vav may represent a new type of signal transduction molecule involved in the transduction of tyrosine phosphorylation signaling into transcriptional events. Vav2 is a member of the Vav family of oncoproteins and acts as a guanosine nucleotide exchange factor (GEF) for RhoG and RhoA-like GTPases in a phosphotyrosine-dependent manner. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Vav2 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, porcine and avian | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 131-330 mapping near the N-terminus of Vav2 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 131-330 mapping near the N-terminus of Vav2 of human origin |
| Entrez Gene | 7410 |
|---|
Application
Western blot analysis of Vav2 expression in HeLa (A) and A-431 (B) whole cell lysates and mouse brain tissue extract (C).
Application
Immunofluor?escence staining of normal mouse intestine frozen section showing cytoplasmic staining.
Application
Western blot analysis of Vav2 expression in HeLa whole cell lysate.| Application Notes | recommended for detection of Vav2 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, porcine and avian |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
