Application
Immunofluores-cence staining of methanol-fixed HeLa cells showing nuclear and cytoskeletal localization. Antibody tested: MICAL2 (K-14): .Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoskeletal localization.| Product Name | MICAL2 Antibody (K-14) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | MICAL2 (microtubule associated monoxygenase, calponin and LIM domain containing 2), also known as MICAL2PV1 or MICAL2PV2, is a 1,124 amino acid protein that localizes to both the cytoplasm and the cytoskeleton and contains one LIM zinc-binding domain and one calponin-homology domain. Using FAD as a cofactor, MICAL2 interacts with Rab 1B, plexin-A4 and Vimentin and, via these interactions, may play a role in cytoskeletal regulation, specifically at intermediate filaments. MICAL2, which exists as two alternatively spliced isoforms, is expressed at high levels in heart and brain and at lower levels in ovary, skeletal muscle, liver, testis and kidney tissue. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIFELISA |
| recommended for detection of MICAL2 isoforms 1 and 2 of mouse, rat and human origin by WB, IF and ELISA non cross-reactive with other MICAL family members also reactive with additional species, including equine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping within an internal region of MICAL2 of human origin |
|---|---|
| Tissue Specificity | epitope mapping within an internal region of MICAL2 of human origin |
| Entrez Gene | 9645 |
|---|
Application
Immunofluores-cence staining of methanol-fixed HeLa cells showing nuclear and cytoskeletal localization. Antibody tested: MICAL2 (K-14): .
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoskeletal localization.| Application Notes | recommended for detection of MICAL2 isoforms 1 and 2 of mouse, rat and human origin by WB, IF and ELISA non cross-reactive with other MICAL family members also reactive with additional species, including equine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
