Application
Western blot analysis of LIV-1 expression in LNCaP (A) and A-375 (B) whole cell lysates and human prostate tissue extract (C).| Product Name | LIV-1 Antibody (E-20) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | LIV-1 (estrogen-regulated protein), also known as ZIP6 (zinc transporter), Zrt- and Irt-like protein 6 or, in mouse and rat, SLC36A6 (solute carrier family 39 member 6), is expressed as two isoforms. LIV-1 is a multi-pass cell membrane protein that is 749 amino acids in length and is expressed abundantly in breast, prostate, placenta, kidney, pituitary and corpus callosum, as well as in cells derived from various types of cancers affecting the glands, cervix and lungs. LIV-1 is a member of the ZIP transporter protein family which consists of 14 members that transport zinc. LIV-1 transports zinc from its position on the plasma membrane into the cytosol of the cell and contains a histidine-rich transmembrane domain which is thought to bind zinc and aid in its transportation. LIV-1 is thought to be important for zinc uptake in neuroblastoma cells and may also be crucial for maintaining zinc homeostasis, a process which aids in the prevention of cancer and disease. Activated estrogen receptors are thought to regulate LIV-1 expression at the level of transcription, via the mRNA precursor to LIV-1 which associates with estrogen receptors that are activated by growth factors and estradiol. LIV-1 is upregulated in hormone-rich tissue, including breast and cervical cancer, where it is thought to affect cell motility and may play an important role in tumor development and metastasis. Conversely, less aggressive tumors may contain high levels of LIV-1 that could lead to apoptosis, indicating a dual role for LIV-1 in tumor suppression. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of LIV-1 of human origin and SLC39A6 of mouse and rat origin by WB, IP, IF and ELISA also reactive with additional species, including canine, bovine and porcine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping within a cytoplasmic domain of LIV-1 of human origin |
|---|---|
| Tissue Specificity | epitope mapping within a cytoplasmic domain of LIV-1 of human origin |
| Entrez Gene | 25800 |
|---|
Application
Western blot analysis of LIV-1 expression in LNCaP (A) and A-375 (B) whole cell lysates and human prostate tissue extract (C).| Application Notes | recommended for detection of LIV-1 of human origin and SLC39A6 of mouse and rat origin by WB, IP, IF and ELISA also reactive with additional species, including canine, bovine and porcine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
