Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.Application
Western blot analysis of MAO-A expression in non-transfected: (A) and human MAO-A transfected: (B) 293T whole cell lysates.Application
Western blot analysis of MAO-A expression in mouse placenta tissue extract.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human duodenum tissue showing cytoplasmic staining of glandular cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program| Product Name | MAO-A Antibody (H-70) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Monoamine oxidase (MAO) is an enzyme of the mitochondrial outer membrane and catalyzes the oxidative deamination of biogenic amines throughout the body (1). MAO is critical in the neuronal metabolism of catecholamine and indolamine transmitters (2). Cultured skin fibroblasts show both MAO-A and MAO-B and both MAOs differ in molecular structure (1). MAO-A, the primary type in fibroblasts, preferentially degrades serotonin and norepinephrine (3). Only MAO-B is present in platelets and only MAO-A is present in trophoblasts (1). MAO-B, the primary type found not only in platelets but also in the brain of man and other primates, preferentially degrades phenylethylamine and benzylamine (3). MAO has been of particular interest to psychiatry and genetics because of the suggestion that low activity is a ‘genetic marker’ for schizophrenia (4). The genes which encode MAO-A and MAO-B map to human chromosome Xp11.23 (5). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of MAO-A of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 458-527 of MAO-A of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 458-527 of MAO-A of human origin |
| Entrez Gene | 4128 |
|---|
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.
Application
Western blot analysis of MAO-A expression in non-transfected: (A) and human MAO-A transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of MAO-A expression in mouse placenta tissue extract.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human duodenum tissue showing cytoplasmic staining of glandular cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program| Application Notes | recommended for detection of MAO-A of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
