Application
Western blot analysis of COPE expression in non-transfected 293: (A), mouse COPE transfected 293: (B), KNRK (C), MCF7 (D) and HeLa (E) whole cell lysates.| Product Name | COPE Antibody (H-80) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Membrane and vesicular trafficking in the early secretory pathway are mediated by non-Clathrin COP (coat protein) I-coated vesicles. COPI-coated vesicles mediate retrograde transport from the Golgi back to the ER and intra-Golgi transport. The cytosolic precursor of the COPI coat, the heptameric coatomer complex, is composed of two subcomplexes. The first consists of the COPB, COPG, COPD and COPZ subunits (also known as b-, g-, d- and z-COP, respectively), which are distantly homologous to AP Clathrin adaptor subunits. The second consists of the COPA, b'-COP and COPE subunits (also known as a-COP, COPP and e-COP, respectively). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of COPE of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 111-190 mapping to an internal region of COPE of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 111-190 mapping to an internal region of COPE of human origin |
| Entrez Gene | 11316 |
|---|
Application
Western blot analysis of COPE expression in non-transfected 293: (A), mouse COPE transfected 293: (B), KNRK (C), MCF7 (D) and HeLa (E) whole cell lysates.| Application Notes | recommended for detection of COPE of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
