Application
Western blot analysis of TRIP12 expression in HeLa (A), Caki-1 (B), SK-MEL-28 (C) and MIA PaCa-2 (D) whole cell lysates.Application
Western blot analysis of TRIP12 expression in HL-60 nuclear extract.| Product Name | TRIP12 Antibody (E-14) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Thyroid hormone receptors (TRs) are transcription factors that regulate the expression of specific genes in a hormone-dependent manner. TRIP12 (thyroid hormone receptor interactor 12) is a 1,992 amino acid E3 ubiquitin ligase involved in the human ubiquitin fusion degradation (UFD) pathway. TRIP12 also modulates the NEDD8 pathway, a series of steps implicated in signal transduction and cell cycle progression, where it influences APPBP1 degradation by catalyzing its ubiquitination. A member of the UPL family and K-HECT subfamily, TRIP 12 contains one WWE domain and a single HECT (E6AP-type E3 ubiquitin-protein ligase) domain suggested to contain a noncovalent ubiquitin-binding site. Subject to post-translational phosphorylation upon DNA damage, TRIP12 expression is highest in testis and skeletal muscle, and has also been found in heart, spleen, thymus, ovary, placenta, kidney, prostate and peripheral blood leukocytes at lower levels. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of TRIP12 of mouse, rat and human origin by WB, IP, IF and ELISA non cross-reactive with other TRIP family members also reactive with additional species, including equine, canine, bovine and avian | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping near the C-terminus of TRIP12 of human origin |
|---|---|
| Tissue Specificity | epitope mapping near the C-terminus of TRIP12 of human origin |
| Entrez Gene | 9320 |
|---|
Application
Western blot analysis of TRIP12 expression in HeLa (A), Caki-1 (B), SK-MEL-28 (C) and MIA PaCa-2 (D) whole cell lysates.
Application
Western blot analysis of TRIP12 expression in HL-60 nuclear extract.| Application Notes | recommended for detection of TRIP12 of mouse, rat and human origin by WB, IP, IF and ELISA non cross-reactive with other TRIP family members also reactive with additional species, including equine, canine, bovine and avian |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
