Application
Immunofluorescence staining of formalin-fixed Hep G2 cells showing membrane localization.Application
Western blot analysis of PRR5 expression in MCF7 (A), SW480 (B), MDA-MB-231 (C), HeLa (D), HEK293 (E) and Hep G2 (F) whole cell lysates.| Product Name | PRR5 Antibody (S-14) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Proline-rich proteins are often involved in protein-protein interactions and typically act as ligands for SH3, WW and EVH1 domains. PRR5 (Proline-rich protein 5), also known as Protein observed with Rictor-1 (Protor-1), is a 388 amino acid protein that is a subunit of mammalian target of rapamycin complex 2 (mTORC2), a multimeric kinase that is involved in the regulation of the actin cytoskeleton and phosphorylates Akt1, SGK and PKC å. Other proteins that make up mTORC2 include Rictor, MAPKAP-1, FRAP and LST8. PRR5 plays an important role in the modulation of platelet-derived growth factor signaling and in the regulation of PDGFR-∫ expression. Since the gene encoding PRR5 is located in a region that frequently undergoes loss of heterozygosity in breast cancer, it is likely that PRR5 is a tumor suppressor. There are four isoforms of PRR5 that are produced as a result of alternative splicing events. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of PRR5 of mouse, rat and human origin by WB, IP, IF and ELISA non cross-reactive with other PRR5 isoforms also reactive with additional species, including equine, canine, bovine and porcine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping within an internal region of PRR5 of human origin |
|---|---|
| Tissue Specificity | epitope mapping within an internal region of PRR5 of human origin |
| Entrez Gene | 55615 |
|---|
Application
Immunofluorescence staining of formalin-fixed Hep G2 cells showing membrane localization.
Application
Western blot analysis of PRR5 expression in MCF7 (A), SW480 (B), MDA-MB-231 (C), HeLa (D), HEK293 (E) and Hep G2 (F) whole cell lysates.| Application Notes | recommended for detection of PRR5 of mouse, rat and human origin by WB, IP, IF and ELISA non cross-reactive with other PRR5 isoforms also reactive with additional species, including equine, canine, bovine and porcine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
